dc.contributor.author | Mørch, Yrr Asbjørg | |
dc.contributor.author | Sandvig, Ioanna | |
dc.contributor.author | Olsen, Øystein | |
dc.contributor.author | Donati, Ivan | |
dc.contributor.author | Thuen, Marte | |
dc.contributor.author | Skjåk-Bræk, Gudmund | |
dc.contributor.author | Haraldseth, Olav | |
dc.contributor.author | Brekken, Christian | |
dc.date.accessioned | 2019-10-11T06:16:05Z | |
dc.date.available | 2019-10-11T06:16:05Z | |
dc.date.created | 2011-12-20T09:19:58Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Contrast Media & Molecular Imaging. 2012, 7 (2), 265-275. | nb_NO |
dc.identifier.issn | 1555-4309 | |
dc.identifier.uri | http://hdl.handle.net/11250/2621483 | |
dc.description.abstract | The aim of the present study was to test alginate gels of different compositions as a system for controlled release of manganese ions (Mn2+) for application in manganese‐enhanced MRI (MEMRI), in order to circumvent the challenge of achieving optimal MRI resolution without resorting to high, potentially cytotoxic doses of Mn2+. Elemental analysis and stability studies of Mn‐alginate revealed marked differences in ion binding capacity, rendering Mn/Ba‐alginate gels with high guluronic acid content most stable. The findings were corroborated by corresponding differences in the release rate of Mn2+ from alginate beads in vitro using T1‐weighted MRI. Furthermore, intravitreal (ivit) injection of Mn‐alginate beads yielded significant enhancement of the rat retina and retinal ganglion cell (RGC) axons 24 h post‐injection. Subsequent compartmental modelling and simulation of ivit Mn2+ transport and concentration revealed that application of slow release contrast agents can achieve a significant reduction of ivit Mn2+ concentration compared with bolus injection. This is followed by a concomitant increase in the availability of ivit Mn2+ for uptake by RGC, corresponding to significantly increased time constants. Our results provide proof‐of‐concept for the applicability of Mn‐alginate gels as a system for controlled release of Mn2+ for optimized MEMRI application. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Wiley | nb_NO |
dc.title | Mn-alginate gels as a novel system for controlled release of Mn2+ in manganese-enhanced MRI | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 265-275 | nb_NO |
dc.source.volume | 7 | nb_NO |
dc.source.journal | Contrast Media & Molecular Imaging | nb_NO |
dc.source.issue | 2 | nb_NO |
dc.identifier.doi | 10.1002/cmmi.493 | |
dc.identifier.cristin | 870575 | |
dc.description.localcode | Copyright © 2012 John Wiley & Sons, Ltd. Open Access | nb_NO |
cristin.unitcode | 194,66,15,0 | |
cristin.unitcode | 194,65,25,0 | |
cristin.unitcode | 1920,4,0,0 | |
cristin.unitname | Institutt for bioteknologi og matvitenskap | |
cristin.unitname | Institutt for sirkulasjon og bildediagnostikk | |
cristin.unitname | Klinikk for bildediagnostikk | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |