dc.contributor.author | Dragset, Marte Singsås | |
dc.contributor.author | Barczak, Amy K. | |
dc.contributor.author | Kannan, Nisha | |
dc.contributor.author | Mærk, Mali | |
dc.contributor.author | Flo, Trude Helen | |
dc.contributor.author | Valla, Svein | |
dc.contributor.author | Rubin, Eric J. | |
dc.contributor.author | Steigedal, Magnus | |
dc.date.accessioned | 2015-09-09T08:11:44Z | |
dc.date.accessioned | 2015-10-28T15:35:52Z | |
dc.date.available | 2015-09-09T08:11:44Z | |
dc.date.available | 2015-10-28T15:35:52Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | PLoS ONE 2015 | nb_NO |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/11250/2358404 | |
dc.description.abstract | Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we
adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to
control inducible gene expression in Mycobacterium smegmatis and Mycobacterium tuberculosis,
the causative agent of human tuberculosis. By making simple changes to a Gramnegative
broad-host-range XylS/Pm-regulated gene expression vector, we prove that it is
possible to adapt this well-studied expression system to non-Gram-negative species. With
the benzoic acid-derived inducer m-toluate, we achieve a robust, time- and dose-dependent
reversible induction of Pm-mediated expression in mycobacteria, with low background
expression levels. XylS/Pm is thus an important addition to existing mycobacterial expression
tools, especially when low basal expression is of particular importance. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Public Library of Science | nb_NO |
dc.title | Benzoic Acid-Inducible Gene Expression in Mycobacteria | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | en_GB |
dc.date.updated | 2015-09-09T08:11:44Z | |
dc.source.journal | PLoS ONE | nb_NO |
dc.identifier.doi | 10.1371/journal.pone.0134544 | |
dc.identifier.cristin | 1262778 | |
dc.description.localcode | © 2015 Dragset et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | nb_NO |