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dc.contributor.authorDragset, Marte Singsås
dc.contributor.authorBarczak, Amy K.
dc.contributor.authorKannan, Nisha
dc.contributor.authorMærk, Mali
dc.contributor.authorFlo, Trude Helen
dc.contributor.authorValla, Svein
dc.contributor.authorRubin, Eric J.
dc.contributor.authorSteigedal, Magnus
dc.date.accessioned2015-09-09T08:11:44Z
dc.date.accessioned2015-10-28T15:35:52Z
dc.date.available2015-09-09T08:11:44Z
dc.date.available2015-10-28T15:35:52Z
dc.date.issued2015
dc.identifier.citationPLoS ONE 2015nb_NO
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2358404
dc.description.abstractConditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in Mycobacterium smegmatis and Mycobacterium tuberculosis, the causative agent of human tuberculosis. By making simple changes to a Gramnegative broad-host-range XylS/Pm-regulated gene expression vector, we prove that it is possible to adapt this well-studied expression system to non-Gram-negative species. With the benzoic acid-derived inducer m-toluate, we achieve a robust, time- and dose-dependent reversible induction of Pm-mediated expression in mycobacteria, with low background expression levels. XylS/Pm is thus an important addition to existing mycobacterial expression tools, especially when low basal expression is of particular importance.nb_NO
dc.language.isoengnb_NO
dc.publisherPublic Library of Sciencenb_NO
dc.titleBenzoic Acid-Inducible Gene Expression in Mycobacterianb_NO
dc.typeJournal articlenb_NO
dc.typePeer revieweden_GB
dc.date.updated2015-09-09T08:11:44Z
dc.source.journalPLoS ONEnb_NO
dc.identifier.doi10.1371/journal.pone.0134544
dc.identifier.cristin1262778
dc.description.localcode© 2015 Dragset et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.nb_NO


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