dc.contributor.author | Hjukse, Jarand Berg | |
dc.contributor.author | Fernández de la Puebla, Mario | |
dc.contributor.author | Vindedal, Gry Fluge | |
dc.contributor.author | Sprengel, Rolf | |
dc.contributor.author | Jensen, Vidar | |
dc.contributor.author | Nagelhus, Erlend Arnulf | |
dc.contributor.author | Tang, Wannan | |
dc.date.accessioned | 2023-12-18T08:40:05Z | |
dc.date.available | 2023-12-18T08:40:05Z | |
dc.date.created | 2023-09-01T10:42:48Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0894-1491 | |
dc.identifier.uri | https://hdl.handle.net/11250/3107936 | |
dc.description.abstract | Astrocytes are intricately involved in the activity of neural circuits; however, their basic physiology of interacting with nearby neurons is not well established. Using two-photon imaging of neurons and astrocytes during higher frequency stimulation of hippocampal CA3-CA1 Schaffer collateral (Scc) excitatory synapses, we could show that increasing levels of released glutamate accelerated local astrocytic Ca2+ elevation. However, blockage of glutamate transporters did not abolish this astrocytic Ca2+ response, suggesting that astrocytic Ca2+ elevation is indirectly associated with an uptake of extracellular glutamate. However, during the astrocytic glutamate uptake, the Na+/Ca2+ exchanger (NCX) reverse mode was activated, and mediated extracellular Ca2+ entry, thereby triggering the internal release of Ca2+. In addition, extracellular Ca2+ entry via membrane P2X receptors further facilitated astrocytic Ca2+ elevation via ATP binding. These findings suggest a novel mechanism of activity induced Ca2+ permeability increases of astrocytic membranes, which drives astrocytic responses during neuronal stimulation of CA3-CA1 Scc excitatory synapses. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley Periodicals LLC. | en_US |
dc.rights | Navngivelse-Ikkekommersiell 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/deed.no | * |
dc.title | Increased membrane Ca<sup>2+</sup> permeability drives astrocytic Ca<sup>2+</sup> dynamics during neuronal stimulation at excitatory synapses | en_US |
dc.title.alternative | Increased membrane Ca<sup>2+</sup> permeability drives astrocytic Ca<sup>2+</sup> dynamics during neuronal stimulation at excitatory synapses | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 2770-2781 | en_US |
dc.source.volume | 71 | en_US |
dc.source.journal | Glia | en_US |
dc.source.issue | 12 | en_US |
dc.identifier.doi | 10.1002/glia.24450 | |
dc.identifier.cristin | 2171571 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |