Protein-protein interactions of the nuclear receprot NR4A2
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The Gastroenterology and Molecular cell biology research group examines biological mechanisms that are important in the development of gastric cancer. The peptide hormone gastrin is known to play a role in organization and maintenance of the gastric epithelium, however, its role in carcinogenesis is debated. Gastrin induces the expression of the nuclear transcription factor NR4A2. NR4A2 expression has been suggested as prognostic factors and potential therapeutic targets for patients with different cancer types, including gastric cancer. The biological functions of NR4A2 in tumors and the prognostic significance are incomplete and needs to be further studied. Recent studies indicate that NR4A2 protein-protein interactions as well as its subcellular localization are of vital importance when it comes to the specific cellular response. The aim of this study was to characterize interacting partners of NR4A2 in cellular model systems. In the present study, the expression of the CCK2 receptor and gastrin induced NR4A2 expression in gastric carcinoma cell lines were examined. The MKN45 and HEK293 cells were used to characterize protein-protein interactions of NR4A2. No interactions were observed between NR4A2 and the proteins initially hypothesized to bind to NR4A2. However, Mass Spectrometry in combination with immunoprecipitation identified several potential binding partners. This thesis provides an overview of potential interactions partners of NR4A2 that may be important in gastric cancer and can provide candidate proteins for further work.