dc.contributor.author | Skjesol, Astrid | |
dc.contributor.author | Yurchenko, Mariya | |
dc.contributor.author | Bösl, Korbinian | |
dc.contributor.author | Gravastrand, Caroline S. | |
dc.contributor.author | Nilsen, Kaja Elisabeth | |
dc.contributor.author | Grøvdal, Lene Melsæther | |
dc.contributor.author | Agliano, Federica | |
dc.contributor.author | Patane, Francesco | |
dc.contributor.author | Lentini, Germana | |
dc.contributor.author | Kim, Hera | |
dc.contributor.author | Teti, Giuseppe | |
dc.contributor.author | Sharma, Aditya Kumar | |
dc.contributor.author | Kandasamy, Richard Kumaran | |
dc.contributor.author | Sporsheim, Bjørnar | |
dc.contributor.author | Starheim, Kristian K. | |
dc.contributor.author | Golenbock, Douglas T. | |
dc.contributor.author | Stenmark, Harald | |
dc.contributor.author | McCaffrey, Mary | |
dc.contributor.author | Espevik, Terje | |
dc.contributor.author | Husebye, Harald | |
dc.date.accessioned | 2021-09-02T07:38:37Z | |
dc.date.available | 2021-09-02T07:38:37Z | |
dc.date.created | 2019-04-09T15:48:07Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | PLoS Pathogens. 2019, 15:e1007684 (3), 1-30. | en_US |
dc.identifier.issn | 1553-7366 | |
dc.identifier.uri | https://hdl.handle.net/11250/2772375 | |
dc.description.abstract | Phagocytosis is a complex process that eliminates microbes and is performed by specialised cells such as macrophages. Toll-like receptor 4 (TLR4) is expressed on the surface of macrophages and recognizes Gram-negative bacteria. Moreover, TLR4 has been suggested to play a role in the phagocytosis of Gram-negative bacteria, but the mechanisms remain unclear. Here we have used primary human macrophages and engineered THP-1 monocytes to show that the TLR4 sorting adapter, TRAM, is instrumental for phagocytosis of Escherichia coli as well as Staphylococcus aureus. We find that TRAM forms a complex with Rab11 family interacting protein 2 (FIP2) that is recruited to the phagocytic cups of E. coli. This promotes activation of the actin-regulatory GTPases Rac1 and Cdc42. Our results show that FIP2 guided TRAM recruitment orchestrates actin remodelling and IRF3 activation, two events that are both required for phagocytosis of Gram-negative bacteria. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | The TLR4 adaptor TRAM controls the phagocytosis of Gram-negative bacteria by interacting with the Rab11-family interacting protein 2 | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 1-30 | en_US |
dc.source.volume | 15:e1007684 | en_US |
dc.source.journal | PLoS Pathogens | en_US |
dc.source.issue | 3 | en_US |
dc.identifier.doi | 10.1371/journal.ppat.1007684 | |
dc.identifier.cristin | 1691186 | |
dc.relation.project | Norges forskningsråd: 262652 | en_US |
dc.relation.project | Norges forskningsråd: 245928 | en_US |
dc.relation.project | St Olavs Hospital HF: 46082500 FFU | en_US |
dc.relation.project | Norges forskningsråd: 223255 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |