Show simple item record

dc.contributor.authorGierman, Lobke
dc.contributor.authorSilva, Gabriela
dc.contributor.authorPervaiz, Zahra
dc.contributor.authorRakner, Johanne Johnsen
dc.contributor.authorMundal, Siv Boon
dc.contributor.authorThaning, Astrid J.
dc.contributor.authorNervik, Ingunn
dc.contributor.authorElschot, Mattijs
dc.contributor.authorMathew, Seema
dc.contributor.authorThomsen, Liv Cecilie Vestrheim
dc.contributor.authorBjørge, Line
dc.contributor.authorIversen, Ann-Charlotte
dc.identifier.citationJournal of Leukocyte Biology. 2020, 1-11.en_US
dc.description.abstractInflammation and oxidative stress at the maternal‐fetal interface characterize the placental dysfunction that underlies the pregnancy disorder preeclampsia. Specialized fetal trophoblasts directly interact with leukocytes at both sites of the maternal‐fetal interface; the uterine wall decidua; and the placenta. TLR3 has been implicated in the harmful inflammation at the maternal‐fetal interface in preeclampsia, but the cellular involvement in the decidua and placenta has not been determined. This study aimed to characterize and quantify cell‐specific TLR3 expression and function at the maternal‐fetal interface in normal and preeclamptic pregnancies. TLR3 expression was assessed by immunohistochemistry and quantified by a novel image‐based and cell‐specific quantitation method. TLR3 was expressed at the maternal‐fetal interface by all decidual and placental trophoblast types and by maternal and fetal leukocytes. Placental, but not decidual, TLR3 expression was significantly higher in preeclampsia compared to normal pregnancies. This increase was attributed to placental intravillous tissue and associated with both moderate and severe placental dysfunction. TLR3 pathway functionality in the decidua and placenta was confirmed by TLR3 ligand‐induced cytokine response, but the TLR3 expression levels did not correlate between the two sites. In conclusion, functional TLR3 was broadly expressed by maternal and fetal cells at both sites of the maternal‐fetal interface and the placental intravillous expression was increased in preeclampsia. This suggests TLR3‐mediated inflammatory involvement with local regulation at both sites of the maternal‐fetal interface in normal and preeclamptic pregnancies.en_US
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.titleTLR3 expression by maternal and fetal cells at the maternal-fetal interface in normal and preeclamptic pregnanciesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalJournal of Leukocyte Biologyen_US
dc.relation.projectNorges forskningsråd: 223255en_US
dc.description.localcodeThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. © 2020 The Authors. Journal of Leukocyte Biology published byWiley Periodicals LLC on behalf of Society for Leukocyte Biologyen_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Navngivelse-Ikkekommersiell 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse-Ikkekommersiell 4.0 Internasjonal