Vis enkel innførsel

dc.contributor.authorOlaisen, Maya
dc.contributor.authorSpigset, Olav
dc.contributor.authorFlatberg, Arnar
dc.contributor.authorGranlund, Atle van Beelen
dc.contributor.authorBrede, wenche Rødseth
dc.contributor.authorAlbrektsen, Grethe
dc.contributor.authorRøyset, Elin Synnøve
dc.contributor.authorGilde, Bodil
dc.contributor.authorSandvik, Arne Kristian
dc.contributor.authorMartinsen, Tom Christian
dc.contributor.authorFossmark, Reidar
dc.date.accessioned2020-02-03T10:18:10Z
dc.date.available2020-02-03T10:18:10Z
dc.date.created2019-09-14T12:27:12Z
dc.date.issued2019
dc.identifier.citationAlimentary Pharmacology and Therapeutics. 2019, 49 (10), 1301-1313.nb_NO
dc.identifier.issn0269-2813
dc.identifier.urihttp://hdl.handle.net/11250/2639227
dc.description.abstractBackground 5‐aminosalicylic acid (5‐ASA) is the first‐line therapy for ulcerative colitis (UC). 5‐ASA acts locally in the colonic mucosa by numerous proposed mechanisms, and is metabolised by N‐acetyltransferase (NAT). Large variations in mucosal 5‐ASA concentrations have been reported, but the underlying mechanisms are not understood. Aim To study the relationship between 5‐ASA concentration, 5‐ASA formulation, NAT genotype and bacterial microbiome in patients with UC. Methods Patients with quiescent UC, using monotherapy of Mezavant (n = 18), Asacol (n = 14) or Pentasa (n = 10), 4.0‐4.8 g/day were included. 5‐ASA was measured in colonic mucosal biopsies and serum by ultra‐high performance liquid chromatography. NAT genotypes were determined by Sanger sequencing. Bacterial microbiome was sequenced from faeces and mucosa by 16S rRNA sequencing using Illumina Miseq. Results Mezavant provided the highest mucosal 5‐ASA levels (geometric mean 2.39 ng/mg), followed by Asacol (1.60 ng/mg, 33% lower, P = 0.50) and Pentasa (0.57 ng/mg, 76% lower, P = 0.033). Mucosal 5‐ASA concentration was not associated with NAT genotype, but serum 5‐ASA concentration and NAT1 genotype was associated (P = 0.044). Mucosal 5‐ASA concentration was positively associated with mucosal bacterial diversity (P = 0.0005) and bacterial composition. High mucosal 5‐ASA concentration was related to reduced abundance of pathogenic bacteria such as Proteobacteria, and increased abundance of several favourable bacteria such as Faecalibacterium. Conclusions Mucosal 5‐ASA concentration is positively associated with bacterial diversity and a mucosal bacterial composition that are perceived favourable in UC. Mezavant yielded higher mucosal 5‐ASA concentrations than Pentasa. 5‐ASA may have beneficial effects on the mucosal microbiome, and high concentrations possibly amend dysbiosis in UC.nb_NO
dc.language.isoengnb_NO
dc.publisherJohn Wiley & Sonsnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleMucosal 5-aminosalicylic acid concentration, drug formulation and mucosal microbiome in patients with quiescent ulcerative colitisnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber1301-1313nb_NO
dc.source.volume49nb_NO
dc.source.journalAlimentary Pharmacology and Therapeuticsnb_NO
dc.source.issue10nb_NO
dc.identifier.doi10.1111/apt.15227
dc.identifier.cristin1724673
dc.relation.projectNorges forskningsråd: 262549nb_NO
dc.description.localcode© 2019 The Authors. Alimentary Pharmacology & Therapeutics Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.nb_NO
cristin.unitcode1920,15,0,0
cristin.unitcode194,65,15,0
cristin.unitcode1920,14,0,0
cristin.unitcode194,65,20,0
cristin.unitnameMedisinsk klinikk
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.unitnameLaboratoriemedisinsk klinikk
cristin.unitnameInstitutt for samfunnsmedisin og sykepleie
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Navngivelse 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Navngivelse 4.0 Internasjonal