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dc.contributor.authorSanchez, Nicolas
dc.contributor.authorBrown, Eunice Nana Adjoa
dc.contributor.authorOlsen, Yngvar
dc.contributor.authorVadstein, Olav
dc.contributor.authorIriarte, Jose Luis
dc.contributor.authorGonzalez, Humberto E.
dc.contributor.authorArdelan, Murat Van
dc.identifier.citationFrontiers in Marine Science. 2018, 5 (118), .nb_NO
dc.description.abstractOrganic ligands play a key role controlling trace metal bioavailability in the world's oceans, yet the species-specific requirements determining whether certain iron forms can be metabolized largely remain unclear. Siderophores are considered relevant within the pool of ligands keeping iron soluble. We used desferrioxamine B (DFB) to study the siderophore's effect on cultures of Skeletonema costatum and Alexandrium catenella. The experimental approach used semi-continuous additions of iron(II) and DFB over time, reaching final concentrations of 1 and 10 nM Fe and 10–10,000 nM DFB. The negative effect of DFB on growth in S. costatum was evident and sharp until day 9 for treatments above 500 nM. Delayed growth occurred at 10,000 nM, reaching ~80% of cell density in Controls under both iron conditions. Alexandrium catenella exhibited a less severe negative effect of DFB on growth, only significant at 10,000 nM, while growth was enhanced at lowest DFB. Total bacterial abundance in diatom and dinoflagellate cultures presented inverse trends. While negatively correlated to DFB in diatom cultures, bacteria showed highest abundances in high DFB treatments in dinoflagellate cultures. Delayed growth exhibited in S. costatum at the highest DFB, indicates that favorable changes for Fe uptake occurred over time, suggesting the involvement of other mechanisms facilitating the diatom cell membrane reduction. Overall, unaffected growth in A. catenella suggests that this species can use FeDFB and therefore has the capacity to access strongly complexed Fe sources. Contrasting responses in the bacterial community associated with each species highlight the complexity of these interactions, while suggesting that for A. catenella it may represent an advantage for acquiring Fe. These results demonstrated the capacity for different uptake strategies among phytoplankton species of different functional groups and underlines the necessity to broaden the study of iron bioavailability on a species basis, alongside interaction with other microbial components such bacteria, to reflect interactions in natural ecosystems.nb_NO
dc.publisherFrontiers Medianb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleEffect of Siderophore on Iron Availability in a Diatom and a Dinoflagellate Species: Contrasting Response in Associated Bacterianb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalFrontiers in Marine Sciencenb_NO
dc.description.localcodeCopyright © 2018 Sanchez, Brown, Olsen, Vadstein, Iriarte, Gonzalez and Ardelan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.nb_NO
cristin.unitnameInstitutt for kjemi
cristin.unitnameInstitutt for biologi
cristin.unitnameInstitutt for bioteknologi og matvitenskap

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