dc.contributor.author | Chiorazzo, MG | |
dc.contributor.author | Tunset, Hanna Maja | |
dc.contributor.author | Popov, AV | |
dc.contributor.author | Johansen, Berit | |
dc.contributor.author | Moestue, Siver Andreas | |
dc.contributor.author | Delikatny, EJ | |
dc.date.accessioned | 2019-04-24T12:15:26Z | |
dc.date.available | 2019-04-24T12:15:26Z | |
dc.date.created | 2018-12-10T09:01:29Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/11250/2595290 | |
dc.description.abstract | Cytosolic phospholipase A2α (cPLA2α) has been shown to be elevated in breast cancer and is a potential biomarker in the differentiation of molecular sub-types. Using a cPLA2α activatable fluorophore, DDAO arachidonate, we explore its ability to function as a contrast agent in fluorescence-guided surgery. In cell lines ranging in cPLA2α expression and representing varying breast cancer sub-types, we show DDAO arachidonate activates with a high correlation to cPLA2α expression level. Using a control probe, DDAO palmitate, in addition to cPLA2α inhibition and genetic knockdown, we show that this activation is a result of cPLA2α activity. In mouse models, using an ex vivo tumor painting technique, we show that DDAO arachidonate activates to a high degree in basal-like versus luminal-like breast tumors and healthy mammary tissue. Finally, we show that using an in vivo model, orthotopic basal-like tumors give significantly high probe activation compared to healthy mammary fat pads and surrounding tissue. Together we conclude that cPLA2α activatable fluorophores such as DDAO arachidonate may serve as a useful contrast agent for the visualization of tumor margins in the fluorescence-guided surgery of basal-like breast cancer. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Springer Nature | nb_NO |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Detection and differentiation of breast cancer sub-types using a cPLA2a activatable fluorophore | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.volume | 9 | nb_NO |
dc.source.journal | Scientific Reports | nb_NO |
dc.source.issue | 1 | nb_NO |
dc.identifier.doi | 10.1038/s41598-019-41626-y | |
dc.identifier.cristin | 1640883 | |
dc.relation.project | Norges forskningsråd: 239940 | nb_NO |
dc.description.localcode | © 2019 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License | nb_NO |
cristin.unitcode | 194,65,25,0 | |
cristin.unitcode | 194,66,10,0 | |
cristin.unitcode | 194,65,15,0 | |
cristin.unitname | Institutt for sirkulasjon og bildediagnostikk | |
cristin.unitname | Institutt for biologi | |
cristin.unitname | Institutt for klinisk og molekylær medisin | |
cristin.ispublished | false | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |