Blar i Institutt for bioteknologi og matvitenskap på dokumenttype "Journal article"
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1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis
(Journal article; Peer reviewed, 2018)The N-terminal domain (residues 28–165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and ... -
1H, 13C, 15N resonance assignment of the apo form of the small, chitin-active lytic polysaccharide monooxygenase JdLPMO10Afrom Jonesia denitrificans
(Peer reviewed; Journal article, 2020)The lytic polysaccharide monooxygenase JdLPMO10A is the N-terminal domain of the multimodular protein Jd1381. The isolated JdLPMO10A domain is one of the smallest chitin-active lytic polysaccharide monooxygenases known to ... -
1H, 13C, 15N resonance assignment of the chitin-active lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis
(Journal article; Peer reviewed, 2015)The chitin-active 19.2 kDa lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis has been isotopically labeled and recombinantly expressed. In this paper, we report the 1H, 13C, 15N resonance assignment ... -
6-Phosphofructokinase and ribulose-5-phosphate 3-epimerase in methylotrophic Bacillus methanolicus ribulose monophosphate cycle
(Journal article; Peer reviewed, 2017)d-Ribulose-5-phosphate-3-epimerase (RPE) and 6-phosphofructokinase (PFK) catalyse two reactions in the ribulose monophosphate (RuMP) cycle in Bacillus methanolicus. The B. methanolicus wild-type strain MGA3 possesses two ... -
A C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides
(Journal article; Peer reviewed, 2014)Lignocellulosic biomass is a renewable resource that significantly can substitute fossil resources for the production of fuels, chemicals, and materials. Efficient saccharification of this biomass to fermentable sugars ... -
A colorimetric assay to rapidly determine the activities of lytic polysaccharide monooxygenases
(Journal article; Peer reviewed, 2018)Background Lytic polysaccharide monooxygenase (LPMOs) are enzymes that catalyze the breakdown of polysaccharides in biomass and have excellent potential for biorefinery applications. However, their activities are relatively ... -
A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli
(Journal article; Peer reviewed, 2013)Abstract Background Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often ... -
A comparative study of diploid versus triploid Atlantic salmon (Salmo salar L.). The effects of rearing temperatures (5, 10 and 15 °C) on raw material characteristics and storage quality
(Journal article; Peer reviewed, 2017)Several major market operators argue that the current level of knowledge about quality is too scant to justify a switch to a large-scale production of triploid salmon. The aim of the present study was, therefore, to elucidate ... -
A comparative study of organic- versus conventional Atlantic salmon. II. Fillet color, carotenoid- and fatty acid composition as affected by dry salting, cold smoking and storage
(Journal article, 2016)The aim of the present study was to investigate the effect of dry salting, cold smoking and 14 days refrigerated storage at 4 °C on the stability of carotenoids, color and fatty acids in commercially reared organic Atlantic ... -
A comparative study of organic- versus conventional farmed Atlantic salmon. I. Pigment and lipid content and composition, and carotenoid stability in ice-stored fillets
(Journal article, 2016)The aim of the present study was to investigate retention of pigments and composition of fatty acids (FA) in farmed organic- and conventional Atlantic salmon fed commercial feed adapted to organic and conventional salmon ... -
A composite network of conserved and tissue specific gene interactions reveals possible genetic interactions in glioma
(Journal article; Peer reviewed, 2017)Differential co-expression network analyses have recently become an important step in the investigation of cellular differentiation and dysfunctional gene-regulation in cell and tissue disease-states. The resulting networks ... -
A correlative spatiotemporal microscale study of calcium phosphate formation and transformation within an alginate hydrogel matrix.
(Journal article; Peer reviewed, 2016)The modification of soft hydrogels with hard inorganic components is a method used to form composite materials with application in non-load-bearing bone tissue engineering. The inclusion of an inorganic component may provide ... -
A new and improved host-independent plasmid system for RK2-based conjugal transfer
(Journal article; Peer reviewed, 2014)Bacterial conjugation is a process that is mediated either by a direct cell-to-cell junction or by formation of a bridge between the cells. It is often used to transfer DNA constructs designed in Escherichia coli to recipient ... -
A non-invasive approach to assess texture changes in sous-vide cooked Atlantic mackerel during chilled storage by fluorescence imaging
(Journal article; Peer reviewed, 2018)The aim of this study was to investigate the potential of fluorescence microscopy coupled with chemical, physical and data analysis methods for reliable and non-invasive detection of changes in texture parameters of sous-vide ... -
A novel antimycobacterial compound acts as an intracellular iron chelator
(Journal article; Peer reviewed, 2015)Efficient iron acquisition is crucial for the pathogenesis of Mycobacterium tuberculosis. Mycobacterial iron uptake and metabolism are therefore attractive targets for antitubercular drug development. Resistance mutations ... -
A novel expression system for lytic polysaccharide monooxygenases
(Journal article; Peer reviewed, 2017)Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive ... -
A novel fluorimetric assay for visualization and quantification of protein carbonyls in muscle foods
(Journal article; Peer reviewed, 2019)Muscle foods, particularly fish products are highly exposed to oxidative stress during processing and storage, resulting in oxidative modification of proteins. Protein carbonyls content has been used as one of the measures ... -
A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector
(Journal article, 2015)The fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen temperature (77 K) provides important insights into the organization of the photosynthetic machinery of bacteria and eukaryotes, which ... -
Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems
(Peer reviewed; Journal article, 2020)Absolute quantification of intracellular metabolite pools is a prerequisite for modeling and in-depth biological interpretation of metabolomics data. It is the final step of an elaborate metabolomics workflow, with challenges ... -
Acid preservation of cultivated brown algae Saccharina latissima and Alaria esculenta and characterization of extracted alginate and cellulose
(Peer reviewed; Journal article, 2023)Cultivated brown algae represent an important potential source of carbohydrate polymers for packaging and other biobased materials. However, their exploitation is currently limited by a short harvest season and a lack of ...