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A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector

Lamb, Jacob Joseph; Forfang, Kristin; Hohmann-Marriott, Martin Frank
Journal article
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URI
http://hdl.handle.net/11250/2372554
Date
2015
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  • Institutt for bioteknologi og matvitenskap [1172]
  • Publikasjoner fra CRIStin - NTNU [26591]
Original version
PLoS ONE 2015, 10(7)   10.1371/journal.pone.0132258
Abstract
The fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen

temperature (77 K) provides important insights into the organization of the photosynthetic

machinery of bacteria and eukaryotes, which cannot be observed at room temperature.

Conventionally, to obtain such spectra, a large and costly table-top fluorometer is required.

Recently portable, reliable, and largely maintenance-free instruments have become available

that can be utilized to accomplish a wide variety of spectroscopy-based measurements

in photosynthesis research. In this report, we show how to build such an instrument in order

to record 77K fluorescence spectra. This instrument consists of a low power monochromatic

light-emitting diode (LED), and a portable CCD array based spectrometer. The optical components

are coupled together using a fiber optic cable, and a custom made housing that

also supports a dewar flask. We demonstrate that this instrument facilitates the reliable

determination of chlorophyll fluorescence emission spectra for the cyanobacterium Synechocystis

sp. PCC 6803, and the green alga Chlamydomonas reinhardtii.
Publisher
Public Library of Science
Journal
PLoS ONE

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