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dc.contributor.authorWesthrin, Marita
dc.contributor.authorXie, Minli
dc.contributor.authorOlderøy, Magnus Ø.
dc.contributor.authorSikorski, Pawel
dc.contributor.authorStrand, Berit Løkensgard
dc.contributor.authorStandal, Therese
dc.date.accessioned2015-09-25T12:23:15Z
dc.date.accessioned2015-10-05T08:39:39Z
dc.date.available2015-09-25T12:23:15Z
dc.date.available2015-10-05T08:39:39Z
dc.date.issued2015
dc.identifier.citationPLoS ONE 2015, 10(3)nb_NO
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2178761
dc.description.abstractMineralized biomaterials are promising for use in bone tissue engineering. Culturing osteogenic cells in such materials will potentially generate biological bone grafts that may even further augment bone healing. Here, we studied osteogenic differentiation of human mesenchymal stem cells (MSC) in an alginate hydrogel systemwhere the cells were coimmobilized with alkaline phosphatase (ALP) for gradual mineralization of the microenvironment. MSC were embedded in unmodified alginate beads and alginate beads mineralized with ALP to generate a polymer/hydroxyapatite scaffold mimicking the composition of bone. The initial scaffold mineralization induced further mineralization of the beads with nanosized particles, and scanning electronmicrographs demonstrated presence of collagen in the mineralized and unmineralized alginate beads cultured in osteogenicmedium. Cells in both types of beads sustained high viability and metabolic activity for the duration of the study (21 days) as evaluated by live/dead staining and alamar blue assay.MSC in beads induced to differentiate in osteogenic direction expressed higher mRNA levels of osteoblast-specific genes (RUNX2, COL1AI, SP7, BGLAP) thanMSC in traditional cell cultures. Furthermore, cells differentiated in beads expressed both sclerostin (SOST) and dental matrix protein-1 (DMP1), markers for late osteoblasts/osteocytes. In conclusion, Both ALP-modified and unmodified alginate beads provide an environment that enhance osteogenic differentiation compared with traditional 2D culture. Also, the ALP-modified alginate beads showed profound mineralization and thus have the potential to serve as a bone substitute in tissue engineering.nb_NO
dc.language.isoengnb_NO
dc.publisherPublic Library of Sciencenb_NO
dc.titleOsteogenic differentiation of human mesenchymal stem cells in mineralized alginate matricesnb_NO
dc.typeJournal articlenb_NO
dc.typePeer revieweden_GB
dc.date.updated2015-09-25T12:23:15Z
dc.source.volume10nb_NO
dc.source.journalPLoS ONEnb_NO
dc.source.issue3nb_NO
dc.identifier.doi10.1371/journal.pone.0120374
dc.identifier.cristin1246232
dc.description.localcode© 2015 Westhrin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.nb_NO


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