Browsing NTNU Open by Author "Aachmann, Finn Lillelund"
Now showing items 41-60 of 81
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Ibuprofen-in-cyclodextrin-in-W/O/W emulsion - Improving the initial and long-term encapsulation efficiency of a model active ingredient.
Hattrem, Magnus; Kristiansen, Kåre Andre; Aachmann, Finn Lillelund; Dille, Morten Johnsen; Draget, Kurt Ingar (Journal article; Peer reviewed, 2015)A challenge in formulating water-in-oil-in-water (W/O/W) emulsions is the uncontrolled release of the encapsulated compound prior to application. Pharmaceuticals and nutraceuticals usually have amphipathic nature, which ... -
Identification of a Pivotal Residue for Determining the Block Structure-Forming Properties of Alginate C-5 Epimerases
Stanisci, Annalucia; Tøndervik, Anne; Gaardløs, Margrethe; Lervik, Anders; Skjåk-Bræk, Gudmund; Sletta, Håvard; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2020)Alginate is a linear copolymer composed of 1→4 linked β-d-mannuronic acid (M) and its epimer α-l-guluronic acid (G). The polysaccharide is first produced as homopolymeric mannuronan and subsequently, at the polymer level, ... -
Innovative Biotechnological Applications of B. subtilis Spore Surface Display for AlgE-type Mannuronan C-5 Epimerases: An NMR-based Study
Stokkan, Lars Thingstad (Master thesis, 2023)Bacillus subtilis (B. subtilis) er anerkjent som modellorganismen for Gram-positive bakterier og har evnen til å danne endosporer når miljøbetingelsene forverres og er mye brukt innen bioteknologi og biokjemi for produksjon ... -
Interactions between carbohydrate-binding modules and carbohydrates – An NMR study
Madland, Eva (Doctoral theses at NTNU;2020:346, Doctoral thesis, 2020)Protein-carbohydrate interactions are vital for all living organisms, whether they are plants, humans, bacteria or viruses. These macromolecular interactions are crucial for processes such as cell-cell communication, ... -
Interactions of a fungal lytic polysaccharide monooxygenase with β-glucan substrates and cellobiose dehydrogenase
Courtade, Gaston; Wimmer, Reinhard; Kjendseth, Åsmund Røhr; Preims, Marita; Felice, Alfons K.G.; Dimarogona, Maria; Vaaje-Kolstad, Gustav; Sørlie, Morten; Sandgren, Mats; Ludwig, Roland; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2016)Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze oxidative cleavage of glycosidic bonds using molecular oxygen and an external electron donor. We have used NMR and isothermal titration ... -
Investigation into the presence of polyphenols and carotenoids in the two seaweed species Alaria esculenta and Saccharina latissima, with a focus on extracting, purifying, and characterizing the xanthophyll fucoxanthin.
Jacobsen, Synnøve Strand (Master thesis, 2021)Kultivering og utnyttelse av tare som en bærekraftig kilde til fornybare ressurser har de siste årene fått mye oppmerksomhet. Forskning på antioksidanter som polyfenoler og karotenoider fra bruntare har spesielt fått mye ... -
Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies
Ellefsen, Christiane Færestrand; Lindstad, Linda; Klau, Leesa Jane; Aachmann, Finn Lillelund; Hiorth, Marianne; Samuelsen, Anne Berit C. (Peer reviewed; Journal article, 2023)Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells of the innate immune system. Although commonly described as (1➔3)-β-glucans with varying degree of (1➔6)-branching, the ... -
Involvement of the ASC-1 complex in processing of methylated DNA and RNA
Rabe, Renana (Doctoral theses at NTNU;2018:83, Doctoral thesis, 2018)Den grunnleggende informasjon om organismene ligger lagret i DNA, som er en polymer sammensatt av fire ulike nitrogenbaser bundet til en sukkerfosfat ryggrad. Langs DNA-tråden ligger ulike gener, og baserekkefølgen innen ... -
Mannuronan C-5 Epimerases Suited for Tailoring of Specific Alginate Structures Obtained by High-Throughput Screening of an Epimerase Mutant Library
Tøndervik, Anne; Klinkenberg, Geir; Aachmann, Finn Lillelund; Svanem, Britt Iren Glærum; Ertesvåg, Helga; Ellingsen, Trond Erling; Valla, Svein; Skjåk-Bræk, Gudmund; Sletta, Håvard (Journal article; Peer reviewed, 2013)The polysaccharide alginate is produced by brown algae and some bacteria and is composed of the two monomers, β-d-mannuronic acid (M) and α-l-guluronic acid (G). The distribution and composition of M/G are important for ... -
Mechanical Properties of Ca-Saturated Hydrogels with Functionalized Alginate
Dalheim, Marianne Øksnes; Omtvedt, Line Aanerud; Bjørge, Isabel M.; Akbarzadeh, Anita; Mano, Joao F.; Aachmann, Finn Lillelund; Strand, Berit Løkensgard (Journal article; Peer reviewed, 2019)In this work, the mechanical properties and stability of alginate hydrogels containing functionalized alginates (peptide and β-cyclodextrin) were studied. There is an increasing interest in the modification of alginates ... -
Mechanistic basis of substrate–O2 coupling within a chitin-active lytic polysaccharide monooxygenase: An integrated NMR/EPR study
Courtade, Gaston; Ciano, Luisa; Paradisi, Alessandro; Lindley, Peter J.; Forsberg, Zarah; Sørlie, Morten; Wimmer, Reinhard; Davies, Gideon J.; Eijsink, Vincent; Walton, Paul H.; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2020)Lytic polysaccharide monooxygenases (LPMOs) have a unique ability to activate molecular oxygen for subsequent oxidative cleavage of glycosidic bonds. To provide insight into the mode of action of these industrially important ... -
Microbial Exopolysaccharides: Evaluation of Glycosyltransferase GumM and 13C Isotope-Labeling of Bacterial Cellulose
Lyngstad, Hera Karlsen (Master thesis, 2021)Mikrobielle eksopolysakkarider er en mangfoldig underkategori av ekstracellulære polymeriske stoffer som utskilles av mikroorganismer. Disse forbindelsene har som regel høy molekylvekt og består av monomerer som er ... -
Mode of action and specificity of a chitinase from unicellular microalgae, Euglena gracilis
Feng, Yiming; Kitaoku, Yoshihito; Tanaka, Jun; Taira, Toki; Ohnuma, Takayuki; Aachmann, Finn Lillelund; Fukamizo, Tamo (Journal article; Peer reviewed, 2018) -
Modification and Characterization of the Catalytic Subunit, the A-module, of the Mannuronan C-5 Epimerases AlgE4 and AlgE6
Dypås, Lene Brattsti (Master thesis, 2015)Alginate is a family of polysaccharides composed of (1 to 4)-linked beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G), with a wide variety of applications and even greater prospects for the future. Alginate is first ... -
Molecular insight into a new low-affinity xylan binding module from the xylanolytic gut symbiont Roseburia intestinalis
Leth, Maria Louise; Ejby, Morten; Madland, Eva; Kitaoku, Yoshihito; Slotboom, Dirk J.; Guskov, Albert; Aachmann, Finn Lillelund; Hachem, Maher Abou (Peer reviewed; Journal article, 2019)Efficient capture of glycans, the prime metabolic resources in the human gut, confers a key competitive advantage for gut microbiota members equipped with extracellular glycoside hydrolases (GHs) to target these substrates. ... -
NMR and fluorescence spectroscopies reveal the preorganized binding site in family 14 carbohydrate-binding module from human chitotriosidase
Madland, Eva; Crasson, Oscar; Vandevenne, Marylene; Sørlie, Morten; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2019)Carbohydrate-binding modules (CBM) play important roles in targeting and increasing the concentration of carbohydrate active enzymes on their substrates. Using NMR to get the solution structure of CBM14, we can gain insight ... -
NMR structure of a lytic polysaccharide monooxygenase provides insight into copper binding, protein dynamics, and substrate interactions
Aachmann, Finn Lillelund; Sørlie, Morten; Skjåk-Bræk, Gudmund; Eijsink, Vincent; Vaaje-Kolstad, Gustav (Journal article; Peer reviewed, 2012)Lytic polysaccharide monooxygenases currently classified as carbohydrate binding module family 33 (CBM33) and glycoside hydrolase family 61 (GH61) are likely to play important roles in future biorefining. However, the ... -
A pair of esterases from a commensal gut bacterium remove acetylations from all positions on complex β-mannans
Michalak, Leszek; Leanti La Rosa, Sabina; Leivers, Shaun Allan; Lindstad, Lars Jordhøy; Kjendseth, Åsmund Røhr; Aachmann, Finn Lillelund; Westereng, Bjørge (Peer reviewed; Journal article, 2020)β-mannans and xylans are important components of the plant cell wall and they are acetylated to be protected from degradation by glycoside hydrolases. β-mannans are widely present in human and animal diets as fiber from ... -
Polysaccharide degradation by lytic polysaccharide monooxygenases
Forsberg, Zarah; Sørlie, Morten; Petrovic, Dejan; Courtade, Gaston; Aachmann, Finn Lillelund; Vaaje-Kolstad, Gustav; Bissaro, Bastien; Kjendseth, Åsmund Røhr; Eijsink, Vincent (Journal article; Peer reviewed, 2019)The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) has had a major impact on our current understanding of the enzymatic conversion of recalcitrant ... -
Preparation of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) and guluronic acid-rich alginate using a unique exo-alginate lyase from Thalassotalea crassostreae
Wang, Damao; Aarstad, Olav Andreas; Li, Jing; McKee, Lauren S.; Sætrom, Gerd Inger; Vyas, Anisha; Srivastava, Vaibhav; Aachmann, Finn Lillelund; Bulone, Vincent; Hsieh, Yves S. Y. (Journal article; Peer reviewed, 2018)Marine multicellular algae are considered promising crops for the production of sustainable biofuels and commodity chemicals. However, their commercial exploitation is currently limited by a lack of appropriate and efficient ...