Blar i NTNU Open på forfatter "Brautaset, Trygve"
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Establishment and validation of the XylS/Pm regulator/promoter system in Streptomyces lividans ΔmatAB for expression and secretion of Lytic Polysaccharide Monooxygenase
Marinovic, Adrian (Master thesis, 2020)Den første generasjonen av biodrivstoff, introdusert som et miljøvennlig alternativ til fossile drivstoff, var laget av matavlinger som er en suboptimal løsning med en verdenspopulasjon som stadig utvider seg slik at ... -
Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus
Irla, Marta Katarzyna; Drejer, Eivind Bøe; Brautaset, Trygve; Hakvåg, Sigrid (Peer reviewed; Journal article, 2020)Background The suitability of bacteria as microbial cell factories is dependent on several factors such as price of feedstock, product range, production yield and ease of downstream processing. The facultative methylotroph ... -
Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by Thermophilic Bacillus methanolicus
Brito, Luciana Fernandes; Irla, Marta Katarzyna; Balzer, Simone; Nærdal, Ingemar; Brautaset, Trygve (Journal article; Peer reviewed, 2021)The use of methanol as carbon source for biotechnological processes has recently attracted great interest due to its relatively low price, high abundance, high purity, and the fact that it is a non-food raw material. In ... -
Evaluation of Pseudomonas putida and Pseudoalteromonas haloplanktis as Alternative Hosts for Recombinant Gene Expression - by Evaluating the xylS/Pm Expression System with mCherry as Reporter Gene
Nygård, Thilde Sofie (Master thesis, 2017)Modern therapeutic proteins, such as insulin, are almost exclusively produced in E. coli. One of the challenges when producing recombinant proteins is solubility: a large fraction of the product is often found in an insoluble ... -
Evaluation of the Pm/XylS expression system for production of IFN-alpha-2b in Cupriavidus necator and Escherichia coli
Bakka, Ingrid Øverlie (Master thesis, 2016)Recombinant bacteria are used for production of human proteins such as the medical important cytokine Interferon-alpha-2b (IFN-α-2b). In the present study, Escheriachia coli (E.coli) and Cupriavidus necator (C.necator) ... -
Evaluation of the XylS/Pm Expression Cassette in Corynebacterium glutamicum
Johnsgaard, Maiken (Master thesis, 2018)For bacteria, the most common expression system for recombinant protein production is Escherichia coli (E. coli). However, a large fraction of recombinant proteins produced in E. coli are in an insoluble form which sometimes ... -
Exploring the Potential of Bacillus methanolicus for Expansion of C1 Substrate Range
Lund, Simen Jervell (Master thesis, 2023)Bruken av den metylotrofe og termofile bakterien B. methanolicus MGA3 som en vert for syntetisk metanotrofi representerer en tiltalende mulighet til å bruke billig metangass som karbonkilde i bioteknologisk produksjon. ... -
Exploring the potential of Corynebacterium glutamicum to produce the novel compatible solute Di-myo-inositol-phosphate
Tonheim, Vilde Andrea (Master thesis, 2022)For å motvirke abiotisk stress, akkumulerer mikroorganismer osmobeskyttende molekyler, også kalt osmolytter, ved enten de novo biosyntese eller opptak fra omgivelsene. Den intracellulære akkumuleringen av disse molekylene med ... -
Exploring the potential of using alternative carbon sources for the fermentative production of riboflavin with engineered Corynebacterium glutamicum
Bakken, Thea Isabel (Master thesis, 2022)B2 -vitaminet, også kjent som riboflavin, er kategorisert som et essensielt næringsstoff for cellene. Mangel på riboflavin vil påvirke de essensielle funksjonene til cellene og forårsake økt risiko for vaskulær sykdom og ... -
From Brown Seaweed to a Sustainable Microbial Feedstock for the Production of Riboflavin
Perez-Garcia, Fernando; Klein, Vivien Jessica; Fernandes de Brito, Luciana; Brautaset, Trygve (Peer reviewed; Journal article, 2022)The increasing global demand for food and energy production encourages the development of new production strategies focused on sustainability. Often, microbial bioprocesses rely on food or feed competitive feedstocks; ... -
Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family
Gawin, Agnieszka Maria; Tietze, Lisa; Aarstad, Olav Andreas; Aachmann, Finn Lillelund; Brautaset, Trygve; Ertesvåg, Helga (Peer reviewed; Journal article, 2020)Bacterial alginate initially consists of 1-4-linked beta-D-mannuronic acid residues (M) which can be later epimerized to alpha-L-guluronic acid (G). The family of AlgE mannuronan C-5-epimerases from Azotobacter vinelandii ... -
Generation of stem cell derived retinal organoids for the disease modeling and treatment of Retinitis Pigmentosa
Schjølberg, Jørn-Ove (Master thesis, 2021)The hereditary retinal disease Retinitis pigmentosa (RP) leads to loss of photoreceptor cells and is a significant cause of blindness, affecting over 2 million worldwide. This project focus on Retinitis pigmentosa type 11 ... -
Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae
Drejer, Eivind Bøe; Hakvåg, Sigrid; Irla, Marta Katarzyna; Brautaset, Trygve (Journal article; Peer reviewed, 2018)Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express ... -
Genome-based genetic tool development for Bacillus methanolicus: Theta-and rolling circle-replicating plasmids for inducible gene expression and application to methanol-based cadaverine production
Irla, Marta; Heggset, Tonje Marita Bjerkan; Nærdal, Ingemar; Paul, Lidia; Haugen, Tone; Le, Simone Balzer; Brautaset, Trygve; Wendisch, Volker F. (Peer reviewed; Journal article, 2016)Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter ... -
Impact of DNA glycosylases on epigenetic regulation of gene expression via active DNA demethylation in human cells
Naderi, Sheida (Master thesis, 2022)Chemical modification of DNA bases is a key strategy employed by cells in epigenetic regulation of gene expression. 5-methylcytosine (5mC) is an important epigenetic mark and transcription repressor in the human genome ... -
Improving expression from the XylS / Pm promoter in Corynebacterium glutamicum
Krakeli, Anne Sofie (Master thesis, 2019)Ved hjelp av rekombinant DNA-teknologi kan gener overføres mellom arter og uttrykkes i andre arter enn opphavsarten. Rekombinante proteinr brukes til en rekke formål i ulike industrier, fra mat- og tekstilindustri til ... -
L-lysine production by Bacillus methanolicus: Genome-based mutational analysis and L-lysine secretion engineering
Nærdal, Ingemar; Netzer, Roman; Irla, Marta; Krog, Anne; Heggset, Tonje Marita Bjerkan; Wendisch, Volker F.; Brautaset, Trygve (Journal article; Peer reviewed, 2017)Bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. This bacterium was previously applied for methanol-based production of l-glutamate, ... -
Metabolic engineering of Bacillus methanolicus for production of recombinant proteins and acetoin from methanol
Drejer, Eivind Bøe (Doctoral theses at NTNU;2020:339, Doctoral thesis, 2020)The use of methanol as a carbon source for industrial biotechnology is a tantalizing prospect. Methanol is highly abundant, and has a long history of efficient, large-scale production. It can be produced abiotically from ... -
Metabolic Engineering of Bacillus methanolicus MGA3 for Production of Acetoin
Chan, Dennis Tin Chat (Master thesis, 2018)Being a pure, inexpensive, non-food chemical that can be sustainably produced, methanol has attracted great interest as a carbon feedstock for microbial production of commodity/specialty chemicals. In this thesis, ...