Evaluation of Pseudomonas putida and Pseudoalteromonas haloplanktis as Alternative Hosts for Recombinant Gene Expression - by Evaluating the xylS/Pm Expression System with mCherry as Reporter Gene
Master thesis
Permanent lenke
http://hdl.handle.net/11250/2441285Utgivelsesdato
2017Metadata
Vis full innførselSamlinger
Sammendrag
Modern therapeutic proteins, such as insulin, are almost exclusively produced in E. coli. One of the challenges when producing recombinant proteins is solubility: a large fraction of the product is often found in an insoluble form, making it less useful for therapeutic applications. The expression technology of Vectron Biosolutions was developed in E. coli, but can cover a broad range of bacterial species. By exploring the use of this technology in different bacterial species, the chances of finding a suitable production process for each protein is enhanced. Project description The goal of this project is to test and further develop the expression technology of Vectron Biosolutions for the production of proteins in alternative bacterial species.