Blar i NTNU Open på forfatter "Brautaset, Trygve"
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Metabolic engineering of Bacillus methanolicus MGA3 for the production of enantiopure 2,3-butanediol
Forseth, Erlend Tvedt (Master thesis, 2023)Metanol som råstoff for mikrobiell produksjon av verdiøkende kjemikalier har flere fordeler i forhold til kostnad og bærekraft. I denne masteroppgaven var målet å lage rekombinante Bacillus methanolicus MGA3 stammer for ... -
Metabolic engineering of Bacillus methanolicus towards methanol-based lactam production
Sørgård, Joakim (Master thesis, 2020)Industriell bioteknologi er en av de største og mest fremtidsrettede feltene som har vokst fram i det 21ste århundret. Det har mange applikasjoner innen viktige industrielle områder som medisin, planteproduksjon, og ... -
Metabolic engineering of industrially relevant strains for the production of riboflavin from sustainable feedstocks
Klein, Vivien Jessica (Doctoral theses at NTNU;2024:95, Doctoral thesis, 2024)There is an increasing demand for agricultural land and other resources necessary to feed an expanding world population. This has led to a growing interest in using sustainable feedstocks to produce value-added compounds ... -
Metabolic Engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol
Klein, Vivien Jessica; Brito, Luciana Fernandes; Perez-Garcia, Fernando; Brautaset, Trygve; Irla, Marta Katarzyna (Peer reviewed; Journal article, 2023)The growing need of next generation feedstocks for biotechnology spurs an intensification of research on the utilization of methanol as carbon and energy source for biotechnological processes. In this paper, we introduced ... -
Metagenomic mapping of bacterial microflora from salmon lice (Lepeophtheirus salmonis) with next-generation sequencing of the 16S ribosomal RNA gene
Hide, Johanne Rekdal (Master thesis, 2019)Lakselusen (Lepeophtheirus salmonis) og fiskesykdommer er store utfordringer innen akvakultur. I denne studien ble den bakterielle mikrofloraen til L. salmonis, samlet fra åtte måneder, kartlagt for å finne informasjon om ... -
Methane monooxygenases: central enzymes in methanotrophy with promising biotechnological applications
Khider, May Laura Kilano; Brautaset, Trygve; Irla, Marta Katarzyna (Peer reviewed; Journal article, 2021)Worldwide, the use of methane is limited to generating power, electricity, heating, and for production of chemicals. We believe this valuable gas can be employed more widely. Here we review the possibility of using methane ... -
Methanol as a carbon substrate in the bio-economy: metabolic engineering of aerobic methylotrophic bacteria for production of value-added chemicals
Pfeifenschneider, Johannes; Brautaset, Trygve; Wendisch, Volker F. (Journal article; Peer reviewed, 2017)Bacteria are widely used as cell factories for production of enzymes and chemicals, mostly from sugars. Methylotrophic bacteria can utilize the one-carbon compound methanol as sole carbon source for growth, and metabolic ... -
Methanol-based acetoin production by genetically engineered Bacillus methanolicus
Drejer, Eivind Bøe; Chan, Dennis; Haupka, Carsten; Wendisch, Volker F.; Brautaset, Trygve; Irla, Marta Katarzyna (Journal article; Peer reviewed, 2019)Methanol is an attractive alternative non-food feedstock for industrial fermentations that can be used instead of sugar-based raw materials. Here, the thermophilic and methylotrophic bacterium Bacillus methanolicus MGA3 ... -
Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains
Nærdal, Ingemar; Pfeifenschneider, Johannes; Brautaset, Trygve; Wendisch, Volker F. (Journal article; Peer reviewed, 2015)Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus ... -
Methanol-based production of heparosan and violacein in Bacillus methanolicus and Methylobacterium extorquens
Ødegård, Sigrid Lyngved (Master thesis, 2021) -
Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains
Irla, Marta; Nærdal, Ingemar; Brautaset, Trygve; Wendisch, Volker F. (Journal article; Peer reviewed, 2017)The use of methanol as a carbon source for biotechnological processes has recently attracted great interest due to relatively low price, high abundance, high purity of methanol, and the fact that it is a non-food raw ... -
Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties
Krog, Anne; Heggeset, Tonje Marita Bjerkan; Müller, Jonas E.N.; Kupper, Christiane E.; Schneider, Olha; Vorholt, Julia A.; Ellingsen, Trond Erling; Brautaset, Trygve (Journal article; Peer reviewed, 2013-03-19)Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD+-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of ... -
Monitoring Parallel Robotic Cultivations with Online Multivariate Analysis
Hans, Sebastian; Ulmer, Christian; Brautaset, Trygve; Neubauer, Peter; Cruz Bournazou, Mariano Nicolas (Peer reviewed; Journal article, 2020)In conditional microbial screening, a limited number of candidate strains are tested at different conditions searching for the optimal operation strategy in production (e.g., temperature and pH shifts, media composition ... -
Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR
Kucharova, Veronika; Aakvik, Trine; Almaas, Eivind; Naas, Adrian Ertsås; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly ... -
Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50C
Hakvåg, Sigrid; Nærdal, Ingemar; Heggeset, Tonje Marita Bjerkan; Kristiansen, Kåre Andre; Aasen, Inga Marie; Brautaset, Trygve (Peer reviewed; Journal article, 2020)The facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate and their derivatives cadaverine and γ-aminobutyric acid (GABA) ... -
Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus
Carnicer, Marc; Vieira, Gilles; Brautaset, Trygve; Portais, Jean-Charles; Heux, Stéphanie (Peer reviewed; Journal article, 2016)Background The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria ... -
Revalorization of agar from red seaweed as microbial feedstock for Corynebacterium glutamicum
Husøy, Ingunn (Master thesis, 2023)I dag er mikrobiell fermentering hovedsakelig basert på bruk av sukker som karbonkilde, hvilket kan virke begrensende når det kommer til bærekraft og konkurrerende bruk av sukker som kilde for menneske- og dyrenæring. ... -
Study and Engineering of Gram- Negative Bacteria as Hosts for Production of Recombinant Proteins and Biopolymers
Gawin, Agnieszka (Doctoral theses at NTNU;2020:173, Doctoral thesis, 2020)Gram-negative bacteria are a large group of prokaryotic organisms that thrive in diverse environments and differ in terms of structure and metabolism. They are capable of producing a variety of natural compounds and ... -
The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.
Gawin, Agnieszka; Valla, Svein; Brautaset, Trygve (Journal article; Peer reviewed, 2017)The XylS/Pm regulator/promoter system originating from the Pseudomonas putida TOL plasmid pWW0 is widely used for regulated low- and high-level recombinant expression of genes and gene clusters in Escherichia coli and other ... -
Transaldolase in Bacillus methanolicus: biochemical characterization and biological role in ribulose monophosphate cycle
Pfeifenschneider, Johannes; Markert, Benno; Stolzenberger, Jessica; Brautaset, Trygve; Wendisch, Volker F. (Peer reviewed; Journal article, 2020)Background The Gram-positive facultative methylotrophic bacterium Bacillus methanolicus uses the sedoheptulose-1,7-bisphosphatase (SBPase) variant of the ribulose monophosphate (RuMP) cycle for growth on the C1 carbon ...