dc.contributor.author | Lamsal, Apsana | |
dc.contributor.author | Andersen, Sonja Benedikte | |
dc.contributor.author | Johansson, Ida | |
dc.contributor.author | Vietri, Marina | |
dc.contributor.author | Bokil, Ansooya Avinash | |
dc.contributor.author | Kurganovs, Natalie Jayne | |
dc.contributor.author | Rylander, Felicia | |
dc.contributor.author | Bjørkøy, Geir | |
dc.contributor.author | Pettersen, Kristine | |
dc.contributor.author | Giambelluca, Miriam Soledad | |
dc.date.accessioned | 2023-07-18T14:41:22Z | |
dc.date.available | 2023-07-18T14:41:22Z | |
dc.date.created | 2023-04-17T12:50:41Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Cell Communication and Signaling. 2023, 21 (1), . | en_US |
dc.identifier.issn | 1478-811X | |
dc.identifier.uri | https://hdl.handle.net/11250/3079730 | |
dc.description.abstract | Background
To our current understanding, solid tumors depend on suppressed local immune reactions, often elicited by the interaction between tumor cells and tumor microenvironment (TME) components. Despite an improved understanding of anti-cancer immune responses in the TME, it is still unclear how immuno-suppressive TME are formed and how some cancer cells survive and metastasize.
Methods
To identify the major adaptations that cancer cells undergo during tumor development and progression, we compared the transcriptome and proteome from metastatic 66cl4 and non-metastatic 67NR cell lines in culture versus their corresponding mouse mammary primary tumors. Using confocal microscopy, RT-qPCR, flow cytometry and western blotting, we studied the signaling pathway and the mechanisms involved. In addition, we used public gene expression data from human breast cancer biopsies to evaluate the correlation between gene expression and clinical outcomes in patients.
Results
We found that type I interferon (IFN-I) response was a key differentially regulated pathway between metastatic and non-metastatic cell lines and tumors. The IFN-I response was active in metastatic cancer cells in culture and markedly dampened when these cells formed primary tumors. Interestingly, the opposite was observed in non-metastatic cancer cells and tumors. Consistent with an active IFN-I response in culture, the metastatic cancer cells displayed elevated levels of cytosolic DNA from both mitochondria and ruptured micronuclei with concomitant activation of cGAS-STING signaling. Interestingly, decreased IFN-I-related gene expression in breast cancer biopsies correlated with an unfavourable prognosis in patients.
Conclusion
Our findings show that IFN-I response is dampened in the tumors with the metastatic ability and lower IFN-I expression predicts poor prognosis in triple-negative and HER2 enriched breast cancer patients. This study highlights the possibility of reactivating the IFN-I response as a potential therapeutic strategy in breast cancer. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | BMC | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Opposite and dynamic regulation of the interferon response in metastatic and non-metastatic breast cancer | en_US |
dc.title.alternative | Opposite and dynamic regulation of the interferon response in metastatic and non-metastatic breast cancer | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 0 | en_US |
dc.source.volume | 21 | en_US |
dc.source.journal | Cell Communication and Signaling | en_US |
dc.source.issue | 1 | en_US |
dc.identifier.doi | 10.1186/s12964-023-01062-y | |
dc.identifier.cristin | 2141271 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |