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dc.contributor.authorSait, Shannen Thora Lea
dc.contributor.authorSurviliene, Vaida
dc.contributor.authorJüssi, Mart
dc.contributor.authorGonzalez, Susana Villa
dc.contributor.authorCiesielski, Tomasz Maciej
dc.contributor.authorJenssen, Bjørn Munro
dc.contributor.authorAsimakopoulos, Alexandros
dc.description.abstractA hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. For most target analytes, the method repeatability ranged from 1.9 to 24% and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method, and 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 12.6–40.1, 7.10–24.2, 0.74–10.7, 1.06–5.72, 0.38–4.38 and <mLOQ - 1.01 ng/mL, respectively. To our knowledge, this is the first study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleDetermination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometryen_US
dc.title.alternativeDetermination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometryen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalTalanta: The International Journal of Pure and Applied Analytical Chemistryen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal