dc.contributor.author | Sait, Shannen Thora Lea | |
dc.contributor.author | Surviliene, Vaida | |
dc.contributor.author | Jüssi, Mart | |
dc.contributor.author | Gonzalez, Susana Villa | |
dc.contributor.author | Ciesielski, Tomasz Maciej | |
dc.contributor.author | Jenssen, Bjørn Munro | |
dc.contributor.author | Asimakopoulos, Alexandros | |
dc.date.accessioned | 2023-01-09T10:20:45Z | |
dc.date.available | 2023-01-09T10:20:45Z | |
dc.date.created | 2023-01-03T13:34:30Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0039-9140 | |
dc.identifier.uri | https://hdl.handle.net/11250/3041889 | |
dc.description.abstract | A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. For most target analytes, the method repeatability ranged from 1.9 to 24% and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method, and 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 12.6–40.1, 7.10–24.2, 0.74–10.7, 1.06–5.72, 0.38–4.38 and <mLOQ - 1.01 ng/mL, respectively. To our knowledge, this is the first study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry | en_US |
dc.title.alternative | Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.volume | 254 | en_US |
dc.source.journal | Talanta: The International Journal of Pure and Applied Analytical Chemistry | en_US |
dc.identifier.doi | 10.1016/j.talanta.2022.124109 | |
dc.identifier.cristin | 2099699 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |