dc.contributor.author | Ahmed, Husnain | |
dc.contributor.author | Stokke, Bjørn Torger | |
dc.date.accessioned | 2022-03-10T09:46:22Z | |
dc.date.available | 2022-03-10T09:46:22Z | |
dc.date.created | 2021-06-04T13:25:58Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Lab on a Chip. 2021, 21 (11), 2232-2243. | en_US |
dc.identifier.issn | 1473-0197 | |
dc.identifier.uri | https://hdl.handle.net/11250/2984192 | |
dc.description.abstract | Micron-sized alginate hydrogel beads are extensively employed as an encapsulation medium for biochemical and biomedical applications. Here we report on the microfluidic assisted fabrication of calcium alginate (Ca-alginate) beads by on-chip picoinjection of aqueous calcium chloride (CaCl2) in emulsified aqueous sodium alginate (Na-alginate) droplets or by picoinjection of Na-alginate solution in emulsified aqueous CaCl2 droplets. There is no added chelator to reduce the Ca activity in either of the two strategies. The two fabrication strategies are implemented using a flow-focusing and picoinjection modules in a single PDMS chip. Aqueous alginate solution was emulsified and infused with CaCl2 solution as the squeezed droplet pass the picoinjection channel; consequently, monodisperse, spherical, and structurally homogeneous Ca-alginate beads were obtained. Monodisperse alginate microgel populations with a mean diameter in the range of 8 to 28 μm and standard deviation less than 1 μm were successfully generated using microfluidic channels with various dimensions and controlling the flow parameters. Monodisperse but also non-spherical particles with diameters 6 to 15 μm were also fabricated when picoinjecting Na-alginate solution in emulsified aqueous CaCl2 droplets. The Ca-alginate microbeads fabricated with tailormade size in the range from sub-cellular and upwards were in both strategies realized without any use of chelators or change in pH conditions, which is considered a significant advantage for further exploitation as encapsulation process for improved enzymatic activity and cell viability. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Royal Society of Chemistry | en_US |
dc.title | Fabrication of monodisperse alginate microgel beads by microfluidic picoinjection: a chelate free approach | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | acceptedVersion | en_US |
dc.rights.holder | This is the authors' accepted manuscript to an article published by Royal Society of Chemistry. Locked until 19.4.2022 due to copyright restrictions. | en_US |
dc.source.pagenumber | 2232-2243 | en_US |
dc.source.volume | 21 | en_US |
dc.source.journal | Lab on a Chip | en_US |
dc.source.issue | 11 | en_US |
dc.identifier.doi | 10.1039/d1lc00111f | |
dc.identifier.cristin | 1913787 | |
cristin.ispublished | true | |
cristin.fulltext | preprint | |
cristin.qualitycode | 1 | |