dc.contributor.author | Aass, Kristin Roseth | |
dc.contributor.author | Mjelle, Robin | |
dc.contributor.author | Kastnes, Martin Haugrud | |
dc.contributor.author | Tryggestad, Synne Stokke | |
dc.contributor.author | van den Brink, Luca M | |
dc.contributor.author | Roseth, Ingrid Aass | |
dc.contributor.author | Westhrin, Marita | |
dc.contributor.author | Zahoor, Muhammad | |
dc.contributor.author | Moen, Siv Helen | |
dc.contributor.author | Nedal, Tonje Marie Vikene | |
dc.contributor.author | Buene, Glenn | |
dc.contributor.author | Misund, Kristine | |
dc.contributor.author | Sponaas, Anne-Marit | |
dc.contributor.author | Ma, Qianli | |
dc.contributor.author | Sundan, Anders | |
dc.contributor.author | Groen, Richard WJ | |
dc.contributor.author | Slørdahl, Tobias Schmidt | |
dc.contributor.author | Waage, Anders | |
dc.contributor.author | Standal, Therese | |
dc.date.accessioned | 2022-02-08T12:27:02Z | |
dc.date.available | 2022-02-08T12:27:02Z | |
dc.date.created | 2022-01-10T16:03:36Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2589-0042 | |
dc.identifier.uri | https://hdl.handle.net/11250/2977713 | |
dc.description.abstract | Interleukin-32 (IL-32) is a nonclassical cytokine expressed in cancers, inflammatory diseases, and infections. Its expression is regulated by two different oxygen sensing systems; HIF1α and cysteamine dioxygenase (ADO), indicating that IL-32 may be involved in the response to hypoxia. We here demonstrate that endogenously expressed, intracellular IL-32 interacts with components of the mitochondrial respiratory chain and promotes oxidative phosphorylation. Knocking out IL-32 in three myeloma cell lines reduced cell survival and proliferation in vitro and in vivo. High-throughput transcriptomic and MS-metabolomic profiling of IL-32 KO cells revealed that cells depleted of IL-32 had perturbations in metabolic pathways, with accumulation of lipids, pyruvate precursors, and citrate. IL-32 was expressed in a subgroup of myeloma patients with inferior survival, and primary myeloma cells expressing IL-32 had a gene signature associated with immaturity, proliferation, and oxidative phosphorylation. In conclusion, we demonstrate a previously unrecognized role of IL-32 in the regulation of plasma cell metabolism. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Cell Press | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.journal | iScience | en_US |
dc.identifier.doi | 10.1016/j.isci.2021.103605 | |
dc.identifier.cristin | 1977779 | |
dc.relation.project | Kreftforeningen: #206643 and #1981161 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |