Blar i Publikasjoner fra CRIStin - NTNU på forfatter "Aachmann, Finn Lillelund"
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1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis
Madland, Eva; Kitaoku, Yoshihito; Sætrom, Gerd Inger; Leth, Maria Louise; Ejby, Morten; Hachem, Maher Abou; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2018)The N-terminal domain (residues 28–165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and ... -
1H, 13C, 15N resonance assignment of the apo form of the small, chitin-active lytic polysaccharide monooxygenase JdLPMO10Afrom Jonesia denitrificans
Christensen, Idd Andrea; Eijsink, Vincent; Aachmann, Finn Lillelund; Courtade, Gaston (Peer reviewed; Journal article, 2020)The lytic polysaccharide monooxygenase JdLPMO10A is the N-terminal domain of the multimodular protein Jd1381. The isolated JdLPMO10A domain is one of the smallest chitin-active lytic polysaccharide monooxygenases known to ... -
1H, 13C, 15N resonance assignment of the chitin-active lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis
Courtade, Gaston; Balzer, Simone; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2015)The chitin-active 19.2 kDa lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis has been isotopically labeled and recombinantly expressed. In this paper, we report the 1H, 13C, 15N resonance assignment ... -
A C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides
Isaksen, Trine; Westereng, Bjørge; Aachmann, Finn Lillelund; Agger, Jane; Kracher, Daniel; Kittl, Roman; Ludwig, R.; Haltrich, Dietmar; Eijsink, Vincent; Horn, Svein Jarle (Journal article; Peer reviewed, 2014)Lignocellulosic biomass is a renewable resource that significantly can substitute fossil resources for the production of fuels, chemicals, and materials. Efficient saccharification of this biomass to fermentable sugars ... -
A colorimetric assay to rapidly determine the activities of lytic polysaccharide monooxygenases
Wang, Damao; Li, Jing; Wong, Ann C. Y.; Aachmann, Finn Lillelund; Hsieh, Yves S. Y. (Journal article; Peer reviewed, 2018)Background Lytic polysaccharide monooxygenase (LPMOs) are enzymes that catalyze the breakdown of polysaccharides in biomass and have excellent potential for biorefinery applications. However, their activities are relatively ... -
A novel expression system for lytic polysaccharide monooxygenases
Courtade, Gaston; Le, Simone Balzer; Sætrom, Gerd Inger; Brautaset, Trygve; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive ... -
Acid preservation of cultivated brown algae Saccharina latissima and Alaria esculenta and characterization of extracted alginate and cellulose
Nøkling-Eide, Katharina; Tan, Fangchang; Wang, Shennan; Zhou, Qi; Gravdahl, Mina; Langeng, Anne-Mari; Bulone, Vincent; Aachmann, Finn Lillelund; Sletta, Håvard; Arlov, Øystein (Peer reviewed; Journal article, 2023)Cultivated brown algae represent an important potential source of carbohydrate polymers for packaging and other biobased materials. However, their exploitation is currently limited by a short harvest season and a lack of ... -
Alginate gels crosslinked with chitosan oligomers - a systematic investigation into alginate block structure and chitosan oligomer interaction
Kopplin, Georg; Lervik, Anders; Draget, Kurt Ingar; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2021)Three alginates with fundamentally different block structures, poly-M, poly-G, and poly-MG, have been investigated upon ionic crosslinking with chitosan oligosaccharides (CHOS), using circular dichroism (CD), rheology, and ... -
Alginate hydrogels functionalized with β-cyclodextrin as a local paclitaxel delivery system
Omtvedt, Line Aanerud; Kristiansen, Kåre Andre; Strand, Wenche Iren; Aachmann, Finn Lillelund; Strand, Berit Løkensgard; Zaytseva-Zotova, Daria Sergeevna (Journal article, 2021)Modification of drug delivery materials with beta-cyclodextrins (β-CyD) is known to increase solubility of poorly water-soluble drugs, protect drugs from degradation and sustain release. In this study, we developed a ... -
Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs)
Westereng, Bjørge; Loose, Jennifer Sarah Maria; Vaaje-Kolstad, Gustav; Aachmann, Finn Lillelund; Sørlie, Morten; Eijsink, Vincent (Journal article; Peer reviewed, 2018)Lytic polysaccharide monooxygenases are copper-dependent enzymes that perform oxidative cleavage of glycosidic bonds in cellulose and various other polysaccharides. LPMOs acting on cellulose use a reactive oxygen species ... -
An assessment of physical and chemical conditions in alginate extraction from two cultivated brown algal species in Norway: Alaria esculenta and Saccharina latissima
Nøkling-Eide, Katharina; Langeng, Anne-Mari; Åslund, Karl Olof Andreas; Aachmann, Finn Lillelund; Sletta, Håvard; Arlov, Øystein (Peer reviewed; Journal article, 2023)Alginates are linear anionic polysaccharides originating from brown algae. Their properties target several application areas in in food-, technical- and pharmaceutical industries, and thus they are highly valued. Norway ... -
Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2
Buchinger, Edith; Wiik, Siv Åshild; Kusnierczyk, Anna; Rabe, Renana; Aas, Per Arne; Kavli, Bodil Merete; Slupphaug, Geir; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Human uracil N-glycosylase isoform 2—UNG2 consists of an N-terminal intrinsically disordered regulatory domain (UNG2 residues 1–92, 9.3 kDa) and a C-terminal structured catalytic domain (UNG2 residues 93–313, 25.1 kDa). ... -
Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C
Courtade, Gaston; Wimmer, Reinhard; Dimarogona, Maria; Sandgren, Mats; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2016)The apo-form of the 23.3 kDa catalytic domain of the AA9 family lytic polysaccharide monooxygenase NcLPMO9C from Neurospora crassa has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, ... -
Bi-functional alginate oligosaccharide–polymyxin conjugates for improved treatment of multidrug-resistant gram-negative bacterial infections
Stokniene, Joana; Powell, Lydia C.; Aarstad, Olav Andreas; Aachmann, Finn Lillelund; Rye, Philip D; Hill, Katja E.; Thomas, David W.; Ferguson, Elaine L. (Peer reviewed; Journal article, 2020)The recent emergence of resistance to colistin, an antibiotic of last resort with dose-limiting toxicity, has highlighted the need for alternative approaches to combat infection. This study aimed to generate and characterise ... -
Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii
Aarstad, Olav Andreas; Stanisci, Annalucia; Sætrom, Gerd Inger; Tøndervik, Anne; Sletta, Håvard; Aachmann, Finn Lillelund; Skjåk-Bræk, Gudmund (Journal article; Peer reviewed, 2019)With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications. Recently, we have shown that alginate from algal ... -
Changes in food quality attributes of Saccharina latissima following pre-treatments, frozen storage and subsequent thawing
Stevant, Pierrick Francois Denis; Aachmann, Finn Lillelund; Arlov, Øystein; Nordtvedt, Tom Ståle; Sarno, Antonio; Aarstad, Olav Andreas; Klau, Leesa Jane; Rustad, Turid; Sæther, Maren; Rebours, Celine (Journal article; Peer reviewed, 2024)Changes in relevant quality attributes for the food industry were monitored in Saccharina latissima following pre-treatment (PT), freezing and thawing. The evaluation included the monitoring of qualitative indicators (dry ... -
Chemical shift assignments for the apo-form of the catalytic domain, the linker region, and the carbohydrate-binding domain of the cellulose-active lytic polysaccharide monooxygenase ScLPMO10C
Courtade, Gaston; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincentius Gerardus Henricus; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)The apo-form of the 21.4 kDa catalytic domain and the 10.7 kDa carbohydrate binding domain of the AA10 family lytic polysaccharide monooxygenase ScLPMO10C from Streptomyces coelicolor have been isotopically labeled and ... -
Chitosan as a wound dressing starting material: antimicrobial Properties and mode of action
Matica, Mariana Adina; Aachmann, Finn Lillelund; Tøndervik, Anne; Sletta, Håvard; Ostafe, Vasile (Journal article; Peer reviewed, 2019)Fighting bacterial resistance is one of the concerns in modern days, as antibiotics remain the main resource of bacterial control. Data shows that for every antibiotic developed, there is a microorganism that becomes ... -
Class IV lasso peptides synergistically induce proliferation of cancer cells and sensitize them to doxorubicin
Madland, Eva; Felipe Guerrero-Garzón, Jaime; Zehl, Martin; Singh, Madhurendra; Rezaei, Shiva; Aachmann, Finn Lillelund; Courtade, Gaston; Urban, Ernst; Rückert, Christian; Busche, Tobias; Kalinowski, Jörn; Cao, Yan-Ru; Jiang, Yi; Jiang, Cheng-lin; Selivanova, Galina; Zotchev, Sergey (Peer reviewed; Journal article, 2020)Heterologous expression of a biosynthesis gene cluster from Amycolatopsis sp. resulted in the discovery of two unique class IV lasso peptides, felipeptins A1 and A2. A mixture of felipeptins stimulated proliferation of ... -
Differential bacterial capture and transport preferences facilitate co-growth on dietary xylan in the human gut
Leth, Maria Louise; Ejby, Morten; Workman, Christopher; Ewald, David Adrian; Pedersen, Signe Schultz; Sternberg, Claus; Bahl, Martin Iain; Licht, Tine Rask; Aachmann, Finn Lillelund; Westereng, Bjørge; Hachem, Maher Abou (Journal article; Peer reviewed, 2018)