Blar i Publikasjoner fra CRIStin - NTNU på forfatter "Aachmann, Finn Lillelund"
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Efficient functionalization of alginate biomaterials
Dalheim, Marianne Øksnes; Vanacker, Julie; Najmi, Maryam A.; Aachmann, Finn Lillelund; Strand, Berit Løkensgard; Christensen, Bjørn E. (Journal article; Peer reviewed, 2016)Peptide coupled alginates obtained by chemical functionalization of alginates are commonly used as scaffold materials for cells in regenerative medicine and tissue engineering. We here present an alternative to the commonly ... -
Efficient Grafting of Cyclodextrin to Alginate and Performance of the Hydrogel for Release of Model Drug
Omtvedt, Line Aanerud; Dalheim, Marianne Øksnes; Nielsen, Thorbjørn T.; Larsen, Kim; Strand, Berit Løkensgard; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2019)Controlling the rate of release of molecules from a hydrogel is of high interest for various drug delivery systems and medical devices. A strategy to alter the release profiles of soluble and poorly soluble active ingredients ... -
Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy
Håti, Armend Gazmeno; Aachmann, Finn Lillelund; Stokke, Bjørn Torger; Skjåk-Bræk, Gudmund; Sletmoen, Marit (Journal article, 2015)Mannuronan C-5 epimerases are a family of enzymes that catalyze epimerization of alginates at the polymer level. This group of enzymes thus enables the tailor-making of various alginate residue sequences to attain various ... -
Enhancement of antimicrobial activity of alginate films with a low amount of carbon nanofibers (0.1% w/w)
Sanmartín-Santos, Isaías; Gandía-Llop, Sofía; Salesa, Beatriz; Martí, Miguel; Aachmann, Finn Lillelund; Serrano-Aroca, Ángel (Journal article; Peer reviewed, 2021) -
Functional characterization of a lytic polysaccharide monooxygenase from Schizophyllum commune that degrades non-crystalline substrates
Østby, Heidi; Christensen, Idd Andrea; Hennum, Karen; Varnai, Aniko; Buchinger, Edith; Grandal, Siri; Courtade, Gaston; Hegnar, Olav Aaseth; Aachmann, Finn Lillelund; Eijsink, Vincent (Journal article; Peer reviewed, 2023)Lytic polysaccharide monooxygenases (LPMOs) are mono-copper enzymes that use O2 or H2O2 to oxidatively cleave glycosidic bonds. LPMOs are prevalent in nature, and the functional variation among these enzymes is a topic of ... -
Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family
Gawin, Agnieszka Maria; Tietze, Lisa; Aarstad, Olav Andreas; Aachmann, Finn Lillelund; Brautaset, Trygve; Ertesvåg, Helga (Peer reviewed; Journal article, 2020)Bacterial alginate initially consists of 1-4-linked beta-D-mannuronic acid residues (M) which can be later epimerized to alpha-L-guluronic acid (G). The family of AlgE mannuronan C-5-epimerases from Azotobacter vinelandii ... -
Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic
Schneider, Olha; Simic, Nebojsa; Aachmann, Finn Lillelund; Rückert, Christian; Kristiansen, Kåre Andre; Kalinowski, Jörn; Jiang, Yi; Wang, Lisong; Jiang, Cheng-Lin; Lale, Rahmi; Zotchev, Sergey B (Journal article; Peer reviewed, 2018)Streptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 ... -
A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus
Kelly, Simon; Hansen, Simon B.; Rübsam, Henriette; Saake, Pia; Pedersen, Emil B.; Gysel, Kira; Madland, Eva; Wu, Shunliang; Wawra, Stephan; Reid, Dugald; Sullivan, John T.; Blahovska, Zuzana; Vinther, Maria; Muszynski, Artur; Azadi, Parastoo; Thygesen, Mikkel B.; Aachmann, Finn Lillelund; Ronson, Clive W.; Zuccaro, Alga; Andersen, Kasper R.; Radutoiu, Simona; Stougaard, Jens (Peer reviewed; Journal article, 2023)Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan ... -
Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity
Frigols, Belen; Marti, Miguel; Salesa, Beatriz; Hernandez-Oliver, Carolina; Aarstad, Olav Andreas; Ulset, Ann-Sissel Teialeret; Sætrom, Gerd Inger; Aachmann, Finn Lillelund; Serrano-Aroca, Angel (Peer reviewed; Journal article, 2019)Alginate is considered an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost in comparison with other biopolymers. We have recently demonstrated that the ... -
Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Crasson, Oscar; Courtade, Gaston; Leonard, Raphael R; Aachmann, Finn Lillelund; Legrand, Francois; Parente, Raffaella; Baurain, Denis; Galleni, Moreno; Sørlie, Morten; Vandevenne, Marylene (Journal article; Peer reviewed, 2017)Chitin is an important structural component of numerous fungal pathogens and parasitic nematodes. The human macrophage chitotriosidase (HCHT) is a chitinase that hydrolyses glycosidic bonds between the N-acetyl-D-glucosamine ... -
Ibuprofen-in-cyclodextrin-in-W/O/W emulsion - Improving the initial and long-term encapsulation efficiency of a model active ingredient.
Hattrem, Magnus; Kristiansen, Kåre Andre; Aachmann, Finn Lillelund; Dille, Morten Johnsen; Draget, Kurt Ingar (Journal article; Peer reviewed, 2015)A challenge in formulating water-in-oil-in-water (W/O/W) emulsions is the uncontrolled release of the encapsulated compound prior to application. Pharmaceuticals and nutraceuticals usually have amphipathic nature, which ... -
Identification of a Pivotal Residue for Determining the Block Structure-Forming Properties of Alginate C-5 Epimerases
Stanisci, Annalucia; Tøndervik, Anne; Gaardløs, Margrethe; Lervik, Anders; Skjåk-Bræk, Gudmund; Sletta, Håvard; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2020)Alginate is a linear copolymer composed of 1→4 linked β-d-mannuronic acid (M) and its epimer α-l-guluronic acid (G). The polysaccharide is first produced as homopolymeric mannuronan and subsequently, at the polymer level, ... -
Interactions of a fungal lytic polysaccharide monooxygenase with β-glucan substrates and cellobiose dehydrogenase
Courtade, Gaston; Wimmer, Reinhard; Kjendseth, Åsmund Røhr; Preims, Marita; Felice, Alfons K.G.; Dimarogona, Maria; Vaaje-Kolstad, Gustav; Sørlie, Morten; Sandgren, Mats; Ludwig, Roland; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2016)Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze oxidative cleavage of glycosidic bonds using molecular oxygen and an external electron donor. We have used NMR and isothermal titration ... -
Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies
Ellefsen, Christiane Færestrand; Lindstad, Linda; Klau, Leesa Jane; Aachmann, Finn Lillelund; Hiorth, Marianne; Samuelsen, Anne Berit C. (Peer reviewed; Journal article, 2023)Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells of the innate immune system. Although commonly described as (1➔3)-β-glucans with varying degree of (1➔6)-branching, the ... -
Mannuronan C-5 Epimerases Suited for Tailoring of Specific Alginate Structures Obtained by High-Throughput Screening of an Epimerase Mutant Library
Tøndervik, Anne; Klinkenberg, Geir; Aachmann, Finn Lillelund; Svanem, Britt Iren Glærum; Ertesvåg, Helga; Ellingsen, Trond Erling; Valla, Svein; Skjåk-Bræk, Gudmund; Sletta, Håvard (Journal article; Peer reviewed, 2013)The polysaccharide alginate is produced by brown algae and some bacteria and is composed of the two monomers, β-d-mannuronic acid (M) and α-l-guluronic acid (G). The distribution and composition of M/G are important for ... -
Mechanical Properties of Ca-Saturated Hydrogels with Functionalized Alginate
Dalheim, Marianne Øksnes; Omtvedt, Line Aanerud; Bjørge, Isabel M.; Akbarzadeh, Anita; Mano, Joao F.; Aachmann, Finn Lillelund; Strand, Berit Løkensgard (Journal article; Peer reviewed, 2019)In this work, the mechanical properties and stability of alginate hydrogels containing functionalized alginates (peptide and β-cyclodextrin) were studied. There is an increasing interest in the modification of alginates ... -
Mechanistic basis of substrate–O2 coupling within a chitin-active lytic polysaccharide monooxygenase: An integrated NMR/EPR study
Courtade, Gaston; Ciano, Luisa; Paradisi, Alessandro; Lindley, Peter J.; Forsberg, Zarah; Sørlie, Morten; Wimmer, Reinhard; Davies, Gideon J.; Eijsink, Vincent; Walton, Paul H.; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2020)Lytic polysaccharide monooxygenases (LPMOs) have a unique ability to activate molecular oxygen for subsequent oxidative cleavage of glycosidic bonds. To provide insight into the mode of action of these industrially important ... -
Mode of action and specificity of a chitinase from unicellular microalgae, Euglena gracilis
Feng, Yiming; Kitaoku, Yoshihito; Tanaka, Jun; Taira, Toki; Ohnuma, Takayuki; Aachmann, Finn Lillelund; Fukamizo, Tamo (Journal article; Peer reviewed, 2018) -
Molecular insight into a new low-affinity xylan binding module from the xylanolytic gut symbiont Roseburia intestinalis
Leth, Maria Louise; Ejby, Morten; Madland, Eva; Kitaoku, Yoshihito; Slotboom, Dirk J.; Guskov, Albert; Aachmann, Finn Lillelund; Hachem, Maher Abou (Peer reviewed; Journal article, 2019)Efficient capture of glycans, the prime metabolic resources in the human gut, confers a key competitive advantage for gut microbiota members equipped with extracellular glycoside hydrolases (GHs) to target these substrates. ... -
NMR and fluorescence spectroscopies reveal the preorganized binding site in family 14 carbohydrate-binding module from human chitotriosidase
Madland, Eva; Crasson, Oscar; Vandevenne, Marylene; Sørlie, Morten; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2019)Carbohydrate-binding modules (CBM) play important roles in targeting and increasing the concentration of carbohydrate active enzymes on their substrates. Using NMR to get the solution structure of CBM14, we can gain insight ...