Blar i Publikasjoner fra CRIStin - NTNU på forfatter "Aachmann, Finn Lillelund"
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NMR structure of a lytic polysaccharide monooxygenase provides insight into copper binding, protein dynamics, and substrate interactions
Aachmann, Finn Lillelund; Sørlie, Morten; Skjåk-Bræk, Gudmund; Eijsink, Vincent; Vaaje-Kolstad, Gustav (Journal article; Peer reviewed, 2012)Lytic polysaccharide monooxygenases currently classified as carbohydrate binding module family 33 (CBM33) and glycoside hydrolase family 61 (GH61) are likely to play important roles in future biorefining. However, the ... -
A pair of esterases from a commensal gut bacterium remove acetylations from all positions on complex β-mannans
Michalak, Leszek; Leanti La Rosa, Sabina; Leivers, Shaun Allan; Lindstad, Lars Jordhøy; Kjendseth, Åsmund Røhr; Aachmann, Finn Lillelund; Westereng, Bjørge (Peer reviewed; Journal article, 2020)β-mannans and xylans are important components of the plant cell wall and they are acetylated to be protected from degradation by glycoside hydrolases. β-mannans are widely present in human and animal diets as fiber from ... -
Polysaccharide degradation by lytic polysaccharide monooxygenases
Forsberg, Zarah; Sørlie, Morten; Petrovic, Dejan; Courtade, Gaston; Aachmann, Finn Lillelund; Vaaje-Kolstad, Gustav; Bissaro, Bastien; Kjendseth, Åsmund Røhr; Eijsink, Vincent (Journal article; Peer reviewed, 2019)The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) has had a major impact on our current understanding of the enzymatic conversion of recalcitrant ... -
Preparation of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) and guluronic acid-rich alginate using a unique exo-alginate lyase from Thalassotalea crassostreae
Wang, Damao; Aarstad, Olav Andreas; Li, Jing; McKee, Lauren S.; Sætrom, Gerd Inger; Vyas, Anisha; Srivastava, Vaibhav; Aachmann, Finn Lillelund; Bulone, Vincent; Hsieh, Yves S. Y. (Journal article; Peer reviewed, 2018)Marine multicellular algae are considered promising crops for the production of sustainable biofuels and commodity chemicals. However, their commercial exploitation is currently limited by a lack of appropriate and efficient ... -
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge
Vuoristo, Kiira; Fredriksen, Lasse; Oftebro, Maren; Arntzen, Magnus Øverlie; Aarstad, Olav Andreas; Stokke, Runar; Steen, Ida Helene; Hansen, Line Degn; Schüller, Reidar Barfod; Aachmann, Finn Lillelund; Horn, Svein Jarle; Eijsink, Vincent (Peer reviewed; Journal article, 2019)Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase ... -
Protective Face Mask Filter Capable of Inactivating SARS-CoV-2, and Methicillin-Resistant Staphylococcus aureus and Staphylococcus epidermidis
Marti, Miguel; Tunon-Molina, Alberto; Aachmann, Finn Lillelund; Muramoto, Yukiko; Noda, Takeshi; Takayama, Kazuo; Serrano-Aroca, Angel (Peer reviewed; Journal article, 2021)Face masks have globally been accepted to be an effective protective tool to prevent bacterial and viral transmission, especially against indoor aerosol transmission. However, commercial face masks contain filters that are ... -
Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A
Kitaoku, Yoshihito; Courtade, Gaston; Petrovic, Dejan; Fukamizo, Tamo; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2018)The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the ... -
RPA2 winged-helix domain facilitates UNG-mediated removal of uracil from ssDNA; implications for repair of mutagenic uracil at the replication fork
Kavli, Bodil Merete; Iveland, Tobias Solli; Buchinger, Edith; Hagen, Lars; Liabakk, Nina-Beate; Aas, Per Arne; Obermann, Tobias Sebastian; Aachmann, Finn Lillelund; Slupphaug, Geir (Journal article; Peer reviewed, 2021)Uracil occurs at replication forks via misincorporation of deoxyuridine monophosphate (dUMP) or via deamination of existing cytosines, which occurs 2-3 orders of magnitude faster in ssDNA than in dsDNA and is 100% miscoding. ... -
Simultaneous analysis of C1 and C4 oxidized oligosaccharides, the products of lytic polysaccharide monooxygenases acting on cellulose
Westereng, Bjørge; Arntzen, Magnus Øverlie; Aachmann, Finn Lillelund; Varnai, Aniko; Eijsink, Vincent; Wittrup Agger, Jane (Journal article; Peer reviewed, 2016)Lytic polysaccharide monooxygenases play a pivotal role in enzymatic deconstruction of plant cell wall material due to their ability to catalyze oxidative cleavage of glycosidic bonds. LPMOs may release different products, ... -
Structural and functional aspects of mannuronic acid–specific PL6 alginate lyase from the human gut microbe Bacteroides cellulosilyticus
Stender, E.G.P.; Andersen, C.D.; Fredslund, F.; Holck, J.; Solberg, Amalie; Teze, D.; Peters, G.H.J.; Christensen, Bjørn E.; Aachmann, Finn Lillelund; Welner, D.H.; Svensson, B. (Journal article; Peer reviewed, 2019)Alginate is a linear polysaccharide from brown algae consisting of 1,4-linked β-D-mannuronic acid (M) and α-L-guluronic acid (G) arranged in M, G, and mixed MG blocks. Alginate was assumed to be indigestible in humans, but ... -
Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii
Buchinger, Edith; Knudsen, Daniel H.; Behrens, Manja A.; Pedersen, Jan Skov; Aarstad, Olav Andreas; Tøndervik, Anne; Valla, Svein; Skjåk-Bræk, Gudmund; Wimmer, Reinhard; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2014)The bacterium Azotobacter vinelandii produces a family of seven secreted and calcium-dependent mannuronan C-5 epimerases (AlgE1–7). These epimerases are responsible for the epimerization of β-d-mannuronic acid (M) to ... -
Structural and functional variation of chitin-binding domains of a lytic polysaccharide monooxygenase from Cellvibrio japonicus
Madland, Eva; Forsberg, Zarah; Wang, Yong; Lindorff-Larsen, Kresten; Niebisch, Axel; Modregger, Jan; Eijsink, Vincent; Aachmann, Finn Lillelund; Courtade, Gaston (Peer reviewed; Journal article, 2021)Among the extensive repertoire of carbohydrate-active enzymes, lytic polysaccharide monooxygenases (LPMOs) have a key role in recalcitrant biomass degradation. LPMOs are copper-dependent enzymes that catalyze oxidative ... -
Synthesis of glycoconjugates utilizing the regioselectivity of a lytic polysaccharide monooxygenase
Westereng, Bjørge; Kračun, SK; Leivers, Shaun Allan; Arntzen, Magnus Øverlie; Aachmann, Finn Lillelund; Eijsink, Vincent (Peer reviewed; Journal article, 2020)Polysaccharides from plant biomass are the most abundant renewable chemicals on Earth and can potentially be converted to a wide variety of useful glycoconjugates. Potential applications of glycoconjugates include therapeutics ... -
The carbohydrate-binding module and linker of a modular lytic polysaccharide monooxygenase promote localized cellulose oxidation
Courtade, Gaston; Forsberg, Zarah; Heggset, Ellinor Bævre; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2018)Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of polysaccharides such as cellulose and chitin, a feature that makes them key tools in industrial biomass ... -
The edible mushroom Albatrellus ovinus contains a α-L-fuco-α-D-galactan, α-D-glucan, a branched (1→6)-β-D-glucan and a branched (1→3)-β-D-glucan
Samuelsen, Anne Berit C.; Rise, Frode; Wilkins, Alistair L.; Teveleva, Lioubov; Nyman, Anna Armika Tussilago; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2019)Albatrellus ovinus, the sheep polypore, is a large, dense mushroom being rich in cell wall material. Polysaccharides were isolated by sequential extraction, enzymatic treatment and analyzed with respect to monosaccharide ... -
Unaided efficient transglutaminase cross-linking of whey proteins strongly impacts the formation and structure of protein alginate particles
Madsen, Mikkel; Khan, Sanaullah; Kunstmann, Sonja; Aachmann, Finn Lillelund; Ipsen, Richard; Westh, Peter; Emanuelsson, Cecilia; Svensson, Birte (Peer reviewed; Journal article, 2022)There is a dogma within whey protein modification, which dictates the necessity of pretreatment to enzymatic cross-linking of β-lactoglobulin (β-Lg). Here microbial transglutaminase (MTG) cross-linked whey proteins and ... -
Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases
Kommedal, Eirik; Angeltveit, Camilla Fløien; Klau, Leesa Jane; Ayuso-Fernandez, Ivan; Arstad, Bjørnar; Antonsen, Simen; Stenstrøm, Yngve; Ekeberg, Dag; Gírio, Francisco; Carvalheiro, Florbela; Horn, Svein Jarle; Aachmann, Finn Lillelund; Eijsink, Vincent (Peer reviewed; Journal article, 2023)Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion of plant biomass in Nature and in industrial applications. Sunlight ...