dc.contributor.author | Hunnestad, Annie Vera | |
dc.contributor.author | Vogel, Anne Ilse Maria | |
dc.contributor.author | Digernes, Maria Guadalupe | |
dc.contributor.author | Armstrong, Evelyn | |
dc.contributor.author | Ardelan, Murat Van | |
dc.contributor.author | Hohmann-Marriott, Martin Frank | |
dc.date.accessioned | 2021-01-27T08:41:41Z | |
dc.date.available | 2021-01-27T08:41:41Z | |
dc.date.created | 2020-11-30T13:41:19Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 2076-2607 | |
dc.identifier.uri | https://hdl.handle.net/11250/2724904 | |
dc.description.abstract | Iron is an essential, yet scarce, nutrient in marine environments. Phytoplankton, and especially cyanobacteria, have developed a wide range of mechanisms to acquire iron and maintain their iron-rich photosynthetic machinery. Iron limitation studies often utilize either oceanographic methods to understand large scale processes, or laboratory-based, molecular experiments to identify underlying molecular mechanisms on a cellular level. Here, we aim to highlight the benefits of both approaches to encourage interdisciplinary understanding of the effects of iron limitation on cyanobacteria with a focus on avoiding pitfalls in the initial phases of collaboration. In particular, we discuss the use of trace metal clean methods in combination with sterile techniques, and the challenges faced when a new collaboration is set up to combine interdisciplinary techniques. Methods necessary for producing reliable data, such as High Resolution Inductively Coupled Plasma Mass Spectrometry (HR-ICP-MS), Flow Injection Analysis Chemiluminescence (FIA-CL), and 77K fluorescence emission spectroscopy are discussed and evaluated and a technical manual, including the preparation of the artificial seawater medium Aquil, cleaning procedures, and a sampling scheme for an iron limitation experiment is included. This paper provides a reference point for researchers to implement different techniques into interdisciplinary iron studies that span cyanobacteria physiology, molecular biology, and biogeochemistry. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | From the Ocean to the Lab—Assessing Iron Limitation in Cyanobacteria: An Interface Paper | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.volume | 8 | en_US |
dc.source.journal | Microorganisms | en_US |
dc.source.issue | 12 | en_US |
dc.identifier.doi | https://doi.org/10.3390/microorganisms8121889 | |
dc.identifier.cristin | 1854171 | |
dc.description.localcode | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |