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dc.contributor.authorMori, Hideki
dc.contributor.authorOgura, Yuko
dc.contributor.authorEnomoto, Kenta
dc.contributor.authorHara, Masayuki
dc.contributor.authorMaurstad, Gjertrud
dc.contributor.authorStokke, Bjørn Torger
dc.contributor.authorKitamura, Shinichi
dc.date.accessioned2020-06-22T09:52:35Z
dc.date.available2020-06-22T09:52:35Z
dc.date.created2020-06-18T11:43:48Z
dc.date.issued2020
dc.identifier.citationPLOS ONE. 2020, 15 1-15.en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/11250/2658984
dc.description.abstractCarbon nanotubes (CNTs) have desirable mechanical properties for use as biomaterials in orthopedic and dental area such as bone- and tooth- substitutes. Here, we demonstrate that a glass surface densely coated with single-walled carbon nanotubes (SWNTs) stimulate the osteogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs). MSCs incubated on SWNT- and multi-walled carbon nanotube (MWNT)-coated glass showed high activities of alkaline phosphatase that are markers for early stage osteogenic differentiation. Expression of Bmp2, Runx2, and Alpl of MSCs showed high level in the early stage for MSC incubation on SWNT- and MWNT-coated surfaces, but only the cells on the SWNT-coated glass showed high expression levels of Bglap (Osteocalcin). The cells on the SWNT-coated glass also contained the most calcium, and their calcium deposits had long needle-shaped crystals. SWNT coating at high density could be part of a new scaffold for bone regeneration.en_US
dc.language.isoengen_US
dc.publisherPublic Library of Science (PLOS)en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleDense carbon-nanotube coating scaffolds stimulate osteogenic differentiation of mesenchymal stem cellsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber1-15en_US
dc.source.volume15en_US
dc.source.journalPLOS ONEen_US
dc.identifier.doi10.1371/journal.pone.0225589
dc.identifier.cristin1816131
dc.description.localcodeCopyright: © 2020 Mori et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal