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dc.contributor.authorArnfinnsdottir, Nina Bjørk
dc.contributor.authorChapman, Cole A
dc.contributor.authorBailey, Ryan C
dc.contributor.authorAksnes, Astrid
dc.contributor.authorStokke, Bjørn Torger
dc.identifier.citationSensors. 2020, 20 (11),en_US
dc.description.abstractRing resonator-based biosensors have found widespread application as the transducing principle in “lab-on-a-chip” platforms due to their sensitivity, small size and support for multiplexed sensing. Their sensitivity is, however, not inherently selective towards biomarkers, and surface functionalization of the sensors is key in transforming the sensitivity to be specific for a particular biomarker. There is currently no consensus on process parameters for optimized functionalization of these sensors. Moreover, the procedures are typically optimized on flat silicon oxide substrates as test systems prior to applying the procedure to the actual sensor. Here we present what is, to our knowledge, the first comparison of optimization of silanization on flat silicon oxide substrates to results of protein capture on sensors where all parameters of two conjugation protocols are tested on both platforms. The conjugation protocols differed in the chosen silanization solvents and protein immobilization strategy. The data show that selection of acetic acid as the solvent in the silanization step generally yields a higher protein binding capacity for C-reactive protein (CRP) onto anti-CRP functionalized ring resonator sensors than using ethanol as the solvent. Furthermore, using the BS3 linker resulted in more consistent protein binding capacity across the silanization parameters tested. Overall, the data indicate that selection of parameters in the silanization and immobilization protocols harbor potential for improved biosensor binding capacity and should therefore be included as an essential part of the biosensor development process.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleImpact of Silanization Parameters and Antibody Immobilization Strategy on Binding Capacity of Photonic Ring Resonatorsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.relation.projectNorges forskningsråd: 248869/O70en_US
dc.relation.projectNorges forskningsråd: 248810en_US
dc.description.localcodeThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal