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dc.contributor.authorLangkilde, Ane
dc.contributor.authorOlsen, Lene Christin
dc.contributor.authorSætrom, Pål
dc.contributor.authorDrabløs, Finn Sverre
dc.contributor.authorBesenbacher, Søren
dc.contributor.authorRaaby, Line
dc.contributor.authorJohansen, Claus
dc.contributor.authorIversen, Lars
dc.date.accessioned2020-04-20T09:01:45Z
dc.date.available2020-04-20T09:01:45Z
dc.date.created2017-01-12T10:37:01Z
dc.date.issued2016
dc.identifier.citationPLOS ONE. 2016, 11 (12), .en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/11250/2651640
dc.description.abstractPsoriasis is a chronic cutaneous inflammatory disease. The immunopathogenesis is a complex interplay between T cells, dendritic cells and the epidermis in which T cells and dendritic cells maintain skin inflammation. Anti-tumour necrosis factor (anti-TNF)-α agents have been approved for therapeutic use across a range of inflammatory disorders including psoriasis, but the anti-inflammatory mechanisms of anti-TNF-α in lesional psoriatic skin are not fully understood. We investigated early events in skin from psoriasis patients after treatment with anti-TNF-α antibodies by use of bioinformatics tools. We used the Human Gene 1.0 ST Array to analyse gene expression in punch biopsies taken from psoriatic patients before and also 4 and 14 days after initiation of treatment with the anti-TNF-α agent adalimumab. The gene expression was analysed by gene set enrichment analysis using the Functional Annotation Tool from DAVID Bioinformatics Resources. The most enriched pathway was visualised by the Pathview Package on Kyoto Encyclopedia of Genes and Genomes (KEGG) graphs. The analysis revealed new very early events in psoriasis after adalimumab treatment. Some of these events have been described after longer periods of anti-TNF-α treatment when clinical and histological changes appear, suggesting that effects of anti-TNF-α treatment on gene expression appear very early before clinical and histological changes. Combining microarray data on biopsies from psoriasis patients with pathway analysis allowed us to integrate in vitro findings into the identification of mechanisms that may be important in vivo. Furthermore, these results may reflect primary effect of anti-TNF-α treatment in contrast to studies of gene expression changes following clinical and histological changes, which may reflect secondary changes correlated to the healing of the skin.en_US
dc.language.isoengen_US
dc.publisherPLOS, Public Library of Scienceen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titlePathway Analysis of Skin from Psoriasis Patients after Adalimumab Treatment Reveals New Early Events in the Anti-Inflammatory Mechanism of Anti-TNF-αen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber16en_US
dc.source.volume11en_US
dc.source.journalPLOS ONEen_US
dc.source.issue12en_US
dc.identifier.doi10.1371/journal.pone.0167437
dc.identifier.cristin1425587
dc.description.localcodeCopyright: © 2016 Langkilde et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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