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dc.contributor.authorAndersen, Maria Karoline
dc.contributor.authorKrossa, Sebastian
dc.contributor.authorHøiem, Therese Stork
dc.contributor.authorBuchholz, Rebecca
dc.contributor.authorClaes, Britt S.R.
dc.contributor.authorBalluff, Benjamin
dc.contributor.authorEllis, Shane
dc.contributor.authorRichardsen, Elin
dc.contributor.authorBertilsson, Helena
dc.contributor.authorHeeren, Ron M.A.
dc.contributor.authorBathen, Tone Frost
dc.contributor.authorKarst, Uwe
dc.contributor.authorGiskeødegård, Guro F.
dc.contributor.authorTessem, May-Britt
dc.identifier.citationAnalytical Chemistry. 2020, 92 (4), 3171-3179.nb_NO
dc.description.abstractLevels of zinc, along with its mechanistically related metabolites citrateand aspartate, are widely reported as reduced in prostate cancer compared to healthytissue and are therefore pointed out as potential cancer biomarkers. Previously, it hasonly been possible to analyze zinc and metabolites by separate detection methods.Through matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI),we were for thefirst time able to demonstrate, in two different sample sets (n= 45 andn= 4), the simultaneous spatial detection of zinc, in the form of ZnCl3−, together withcitrate, aspartate, andN-acetylaspartate on human prostate cancer tissues. The reliabilityof the ZnCl3−detection was validated by total zinc determination using laser ablationinductively coupled plasma MSI on adjacent serial tissue sections. Zinc, citrate, andaspartate were correlated with each other (ranger= 0.46 to 0.74) and showed asignificant reduction in cancer compared to non-cancer epithelium (p< 0.05, log2foldchange range:−0.423 to−0.987), while no significant difference between cancer andstroma tissue was found. Simultaneous spatial detection of zinc and its metabolites is notonly a valuable tool for analyzing the role of zinc in prostate metabolism but might alsoprovide a fast and simple method to detect zinc, citrate, and aspartate levels as abiomarker signature for prostate cancer diagnostics and prognostics.nb_NO
dc.publisherAmerican Chemical Societynb_NO
dc.rightsNavngivelse 4.0 Internasjonal
dc.titleSimultaneous Detection of Zinc and Its Pathway Metabolites Using MALDI MS Imaging of Prostate Tissuenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalAnalytical Chemistrynb_NO
dc.relation.projectEU 758306
dc.description.localcodeThis is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.nb_NO
cristin.unitnameInstitutt for sirkulasjon og bildediagnostikk
cristin.unitnameInstitutt for klinisk og molekylær medisin

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