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Electron Beam Lithography Fabrication of SU-8 Polymer Structures for Cell Studies

Vinje, Jakob; Beckwith, Kai Sandvold; Sikorski, Pawel
Journal article, Peer reviewed
Accepted version
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URI
http://hdl.handle.net/11250/2640127
Date
2020
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  • Institutt for fysikk [1753]
  • Institutt for klinisk og molekylær medisin [2004]
  • Publikasjoner fra CRIStin - NTNU [19743]
Original version
Journal of microelectromechanical systems   10.1109/JMEMS.2020.2967174
Abstract
Flat surfaces decorated with micro- and nanostructures are important tools in biomedical research used to control cellular shape, in studies of mechanotransduction, membrane mechanics, cell migration and cellular interactions with nanostructured surfaces. Existing methods to fabricate surface-bound nanostructures are typically limited either by resolution, aspect ratio or throughput. In this work, we explore electron beam lithography based structuring of the epoxy resist SU-8 on glass substrate. We focus on a systematic investigation of the process parameters and determine limits of the fabrication process, both in terms of spatial resolution, structure aspect ratio and fabrication throughput. The described approach is capable of producing high-aspect ratio, surface bound nanostructures with height ranging from 100 nm to 4000 nm and with in-plane resolution below 100 nm directly on a transparent substrate. Fabricated nanostructured surfaces can be integrated with common techniques for biomedical research, such as high numerical aperture optical microscopy. Furthermore, we show how the described approach can be used to make nanostructures with multiple heights on the same surface, something which is not readily achievable using alternative fabrication approaches. Our research paves an alternative way of manufacturing nanostructured surfaces with applications in life science research. [2019-0255]
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Journal
Journal of microelectromechanical systems

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