dc.contributor.author | Prikrylova, Terezia | |
dc.contributor.author | Robertson, Julia | |
dc.contributor.author | Ferrucci, Francesca | |
dc.contributor.author | Konorska, Dorota Joanna | |
dc.contributor.author | Aanes, Håvard | |
dc.contributor.author | Manaf, Adeel | |
dc.contributor.author | Zhang, Beibei | |
dc.contributor.author | Vågbø, Cathrine Broberg | |
dc.contributor.author | Kusnierczyk, Anna | |
dc.contributor.author | Gilljam, Karin Margaretha | |
dc.contributor.author | Løvkvam-Køster, Caroline | |
dc.contributor.author | Otterlei, Marit | |
dc.contributor.author | Dahl, John Arne | |
dc.contributor.author | Enserink, Jorrit | |
dc.contributor.author | Klungland, Arne | |
dc.contributor.author | Robertson, Adam Brian | |
dc.date.accessioned | 2020-01-13T09:31:24Z | |
dc.date.available | 2020-01-13T09:31:24Z | |
dc.date.created | 2019-09-16T11:16:32Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Scientific Reports. 2019, 9:11065 1-16. | nb_NO |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/11250/2635867 | |
dc.description.abstract | In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower eukaryotes, replication is initiated from a defined consensus sequence, whereas a consensus sequence delineating mammalian origin of replication has not been identified. Here we show that 5-hydroxymethylcytosine (5hmC) is present at mammalian replication origins. Our data support the hypothesis that 5hmC has a role in cell cycle regulation. We show that 5hmC level is inversely proportional to proliferation; indeed, 5hmC negatively influences cell division by increasing the time a cell resides in G1. Our data suggest that 5hmC recruits replication-licensing factors, then is removed prior to or during origin firing. Later we propose that TET2, the enzyme catalyzing 5mC to 5hmC conversion, acts as barrier to rereplication. In a broader context, our results significantly advance the understating of 5hmC involvement in cell proliferation and disease states. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Nature Research | nb_NO |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 1-16 | nb_NO |
dc.source.volume | 9:11065 | nb_NO |
dc.source.journal | Scientific Reports | nb_NO |
dc.identifier.doi | 10.1038/s41598-019-47528-3 | |
dc.identifier.cristin | 1725022 | |
dc.description.localcode | © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) | nb_NO |
cristin.unitcode | 194,65,15,0 | |
cristin.unitname | Institutt for klinisk og molekylær medisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |