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dc.contributor.advisorKentros, Clifford
dc.contributor.advisorBlankvoort, Stefan
dc.contributor.authorFlatset, Marcus Sandbukt
dc.date.accessioned2019-10-09T14:00:05Z
dc.date.available2019-10-09T14:00:05Z
dc.date.issued2019
dc.identifier.urihttp://hdl.handle.net/11250/2621233
dc.description.abstract
dc.description.abstractThe entorhinal cortex is the main gateway between the hippocampal formation and the neocortex. The entorhinal cortex mainly projects to the hippocampal formation via layers II and III. While the various functions of entorhinal cortex layer II has been extensively studied in recent years, the connectivity of entorhinal cortex layer III has remained relatively unstudied. Here we seek to take advantage of novel transgenic tools and viral tracers in order to trace the up‐ and downstream connections of layer III in the medial entorhinal cortex. The combination of methods we use enable us to target a group of cells with unprecedented specificity, allowing accurate identification of local and global projections. Previous work has shown that layer III of the entorhinal cortex projects to CA1 and the subiculum, both ipsilaterally and contralaterally, while the main inputs to layer III are the presubiculum, postrhinal cortex and layer V of the entorhinal cortex. Using cell type specific tracing methods, I find largely similar afferent and efferent connections, but for the first time with layer specificity in mice. I also find that a recently described input from CA2 to layer II of the medial entorhinal cortex could also project to layer III. Additionally, I find support for the more recently described parasubicular input to layer III of the medial entorhinal cortex. Finally, these data show a larger input to MEC LIII from the CA1 than what was expected from traditional tracing methods. These novel findings may be the result of technical artifacts, species differences or previously under‐reported connections. Overall, this thesis shows the structural connectivity to and from MEC LIII in the mouse, and could lead to future functional connectivity studies in the area.
dc.languageeng
dc.publisherNTNU
dc.titleExploring connectivity in layer III of the mouse medial entorhinal cortex using viral tracers and novel transgenic tools
dc.typeMaster thesis


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