dc.contributor.author | Hiseni, Pranvera | |
dc.contributor.author | Wilson, Robert Charles | |
dc.contributor.author | Storrø, Ola | |
dc.contributor.author | Johnsen, Roar | |
dc.contributor.author | Øien, Torbjørn | |
dc.contributor.author | Rudi, Knut | |
dc.date.accessioned | 2019-09-03T13:36:22Z | |
dc.date.available | 2019-09-03T13:36:22Z | |
dc.date.created | 2019-06-11T09:27:53Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | BioTechniques. 2019, 66 (3), 143-149. | nb_NO |
dc.identifier.issn | 0736-6205 | |
dc.identifier.uri | http://hdl.handle.net/11250/2612321 | |
dc.description.abstract | We present a novel liquid array diagnostics (LAD) method, which enables rapid and inexpensive detection of microbial markers in a single-tube multiplex reaction. We evaluated LAD both on pure cultures, and on infant gut microbiota for a 15-plex reaction. LAD showed more than 80% accuracy of classification and a detection limit lower than 2% of the Illumina reads per sample. The results on the clinical dataset showed that there was a rapid decrease of staphylococci from 10-day- to 4-month-old children, a peak of bifidobacteria at 4 months, and a peak of Bacteroides in 2-year-old children, which is in accordance with findings described in the literature. Being able to detect up to 50 biomarkers, LAD is a suitable method for assays where high throughput is essential. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Future Medicine | nb_NO |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | Liquid array diagnostics: A novel method for rapid detection of microbial communities in single-tube multiplex reactions | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 143-149 | nb_NO |
dc.source.volume | 66 | nb_NO |
dc.source.journal | BioTechniques | nb_NO |
dc.source.issue | 3 | nb_NO |
dc.identifier.doi | 10.2144/btn-2018-0134 | |
dc.identifier.cristin | 1703833 | |
dc.description.localcode | This work is licensed under the AttributionNonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/ by-nc-nd/4.0/ | nb_NO |
cristin.unitcode | 194,65,20,0 | |
cristin.unitname | Institutt for samfunnsmedisin og sykepleie | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |