dc.contributor.author | Gregersen, Ida | |
dc.contributor.author | Sandanger, Øystein | |
dc.contributor.author | Askevold, Erik Tandberg | |
dc.contributor.author | Sagen, Ellen Lund | |
dc.contributor.author | Yang, Kuan | |
dc.contributor.author | Holm, Sverre | |
dc.contributor.author | Pedersen, Turid Margrethe | |
dc.contributor.author | Skjelland, Mona | |
dc.contributor.author | Krohg-Sørensen, Kirsten | |
dc.contributor.author | Hansen, Trond Vidar | |
dc.contributor.author | Dahl, Tuva Børresdatter | |
dc.contributor.author | Otterdal, Kari | |
dc.contributor.author | Espevik, Terje | |
dc.contributor.author | Aukrust, Pål | |
dc.contributor.author | Yndestad, Arne | |
dc.contributor.author | Halvorsen, Bente | |
dc.date.accessioned | 2018-01-09T10:14:02Z | |
dc.date.available | 2018-01-09T10:14:02Z | |
dc.date.created | 2017-12-04T13:14:50Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | PLoS ONE. 2017, 12 (11): e0188387. | nb_NO |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/11250/2476381 | |
dc.description.abstract | Aim
Interleukin-27 (IL-27) is involved in different inflammatory diseases; however, its role in atherosclerosis is unclear. In this study we investigated the expression of IL-27 and its receptor in patients with carotid atherosclerosis and if IL-27 could modulate the inflammatory effects of the NLRP3 inflammasome in vitro.
Methods
Plasma IL-27 was measured by enzyme immunoassay in patients with carotid stenosis (n = 140) and in healthy controls (n = 19). Expression of IL-27 and IL-27R was analyzed by quantitative PCR and immunohistochemistry in plaques from patients and in non-atherosclerotic vessels. THP-1 monocytes, primary monocytes and peripheral blood mononuclear cells (PBMCs) were used to study effects of IL-27 in vitro.
Results
Our main findings were: (i) Plasma levels of IL-27 were significantly elevated in patients with carotid atherosclerotic disease compared to healthy controls. (ii) Gene expression of IL-27 and IL-27R was significantly elevated in plaques compared to control vessels, and co-localized to macrophages. (iii) In vitro, IL-27 increased NLRP3 inflammasome activation in monocytes with enhanced release of IL-1 β.
Conclusions
We demonstrate increased levels of IL-27 and IL-27R in patients with carotid atherosclerosis. Our in vitro findings suggest an inflammatory role for IL-27, which can possibly be linked to atherosclerotic disease development. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Public Library of Science | nb_NO |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation. | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.volume | 12 | nb_NO |
dc.source.journal | PLoS ONE | nb_NO |
dc.source.issue | 11 | nb_NO |
dc.identifier.doi | 10.1371/journal.pone.0188387 | |
dc.identifier.cristin | 1522499 | |
dc.description.localcode | © 2017 Gregersen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | nb_NO |
cristin.unitcode | 194,65,15,0 | |
cristin.unitname | Institutt for klinisk og molekylær medisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |