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dc.contributor.authorGregersen, Ida
dc.contributor.authorSandanger, Øystein
dc.contributor.authorAskevold, Erik Tandberg
dc.contributor.authorSagen, Ellen Lund
dc.contributor.authorYang, Kuan
dc.contributor.authorHolm, Sverre
dc.contributor.authorPedersen, Turid Margrethe
dc.contributor.authorSkjelland, Mona
dc.contributor.authorKrohg-Sørensen, Kirsten
dc.contributor.authorHansen, Trond Vidar
dc.contributor.authorDahl, Tuva Børresdatter
dc.contributor.authorOtterdal, Kari
dc.contributor.authorEspevik, Terje
dc.contributor.authorAukrust, Pål
dc.contributor.authorYndestad, Arne
dc.contributor.authorHalvorsen, Bente
dc.date.accessioned2018-01-09T10:14:02Z
dc.date.available2018-01-09T10:14:02Z
dc.date.created2017-12-04T13:14:50Z
dc.date.issued2017
dc.identifier.citationPLoS ONE. 2017, 12 (11): e0188387.nb_NO
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2476381
dc.description.abstractAim Interleukin-27 (IL-27) is involved in different inflammatory diseases; however, its role in atherosclerosis is unclear. In this study we investigated the expression of IL-27 and its receptor in patients with carotid atherosclerosis and if IL-27 could modulate the inflammatory effects of the NLRP3 inflammasome in vitro. Methods Plasma IL-27 was measured by enzyme immunoassay in patients with carotid stenosis (n = 140) and in healthy controls (n = 19). Expression of IL-27 and IL-27R was analyzed by quantitative PCR and immunohistochemistry in plaques from patients and in non-atherosclerotic vessels. THP-1 monocytes, primary monocytes and peripheral blood mononuclear cells (PBMCs) were used to study effects of IL-27 in vitro. Results Our main findings were: (i) Plasma levels of IL-27 were significantly elevated in patients with carotid atherosclerotic disease compared to healthy controls. (ii) Gene expression of IL-27 and IL-27R was significantly elevated in plaques compared to control vessels, and co-localized to macrophages. (iii) In vitro, IL-27 increased NLRP3 inflammasome activation in monocytes with enhanced release of IL-1 β. Conclusions We demonstrate increased levels of IL-27 and IL-27R in patients with carotid atherosclerosis. Our in vitro findings suggest an inflammatory role for IL-27, which can possibly be linked to atherosclerotic disease development.nb_NO
dc.language.isoengnb_NO
dc.publisherPublic Library of Sciencenb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleInterleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation.nb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.volume12nb_NO
dc.source.journalPLoS ONEnb_NO
dc.source.issue11nb_NO
dc.identifier.doi10.1371/journal.pone.0188387
dc.identifier.cristin1522499
dc.description.localcode© 2017 Gregersen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.nb_NO
cristin.unitcode194,65,15,0
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal