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dc.contributor.authorTangerås, Line
dc.contributor.authorStødle, Guro
dc.contributor.authorOlsen, Guro Dalheim
dc.contributor.authorLeknes, Ann-Helen
dc.contributor.authorGundersen, Astrid
dc.contributor.authorSkei, Bente
dc.contributor.authorVikdal, Anne Jorunn
dc.contributor.authorRyan, Liv
dc.contributor.authorSteinkjer, Bjørg
dc.contributor.authorMyklebost, Merete
dc.contributor.authorLangaas, Mette
dc.contributor.authorAustgulen, Rigmor
dc.contributor.authorIversen, Ann-Charlotte
dc.identifier.citationJournal of Reproductive Immunology. 2014, 106 89-99.nb_NO
dc.description.abstractToll-like receptors (TLRs) are an important part of the body's danger response system and crucial for initiating inflammation in response to cellular stress, tissue damage, and infections. Proper placental development is sensitive to inflammatory activation, and a role for TLRs in trophoblast immune activation has been suggested, but no overall examination has been performed in primary trophoblasts of early pregnancy. This study aimed to broadly examine cell surface and endosomal TLR gene expression and activation in first-trimester trophoblasts. Gene expression of all ten TLRs was examined by quantitative RT-PCR (RT-qPCR) in primary first-trimester trophoblasts (n = 6) and the trophoblast cell line BeWo, and cytokine responses to TLR ligands were detected by quantitative multiplex immunoassay. Primary first-trimester trophoblasts broadly expressed all ten TLR mRNAs; TLR1, TLR2, TLR3, TLR4, and TLR6 mRNA were expressed by all primary trophoblast populations, while TLR5, TLR7, TLR8, TLR9, and TLR10 mRNA expression was more restricted. Functional response to ligand activation of cell surface TLR2/1, TLR4, and TLR5 increased IL-6 and/or IL-8 release (P < 0.01) from primary trophoblasts. For endosomal TLRs, TLR3 and TLR9 ligand exposure increased receptor-specific production of IL-8 (P < 0.01) and IFN-γ-induced protein 10 (IP-10; P < 0.001) or vascular endothelial growth factor A (VEGFA; P < 0.01). In contrast, BeWo cells expressed lower TLR mRNA levels and did not respond to TLR activation. In conclusion, primary first-trimester trophoblasts broadly express functional TLRs, with inter-individual variation, suggesting that trophoblast TLR2, TLR3, TLR4, TLR5, and TLR9 might play a role in early placental inflammation.nb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.titleFunctional Toll-like receptors in primary first-trimester trophoblastsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalJournal of Reproductive Immunologynb_NO
dc.relation.projectNorges forskningsråd: 223255nb_NO
dc.relation.projectNorges forskningsråd: 205400nb_NO
dc.description.localcodeThis is the authors' accepted and refereed manuscript to the article.nb_NO
cristin.unitnameInstitutt for kreftforskning og molekylær medisin
cristin.unitnameInstitutt for matematiske fag

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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal