dc.contributor.author | Brembu, Tore | |
dc.contributor.author | Chauton, Matilde Skogen | |
dc.contributor.author | Winge, Per | |
dc.contributor.author | Bones, Atle M. | |
dc.contributor.author | Vadstein, Olav | |
dc.date.accessioned | 2017-09-28T11:18:46Z | |
dc.date.available | 2017-09-28T11:18:46Z | |
dc.date.created | 2017-05-15T23:36:05Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Scientific Reports. 2017, 7 . | nb_NO |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/11250/2457296 | |
dc.description.abstract | The diatom cell wall, or frustule, is a highly complex, three-dimensional structure consisting of nanopatterned silica as well as proteins and other organic components. While some key components have been identified, knowledge on frustule biosynthesis is still fragmented. The model diatom Thalassiosira pseudonana was subjected to silicon (Si) shift-up and shift-down situations. Cellular and molecular signatures, dynamic changes and co-regulated clusters representing the hallmarks of cellular and molecular responses to changing Si availabilities were characterised. Ten new proteins with silaffin-like motifs, two kinases and a novel family of putatively frustule-associated transmembrane proteins induced by Si shift-up with a possible role in frustule biosynthesis were identified. A separate cluster analysis performed on all significantly regulated silaffin-like proteins (SFLPs), as well as silaffin-like motifs, resulted in the classification of silaffins, cingulins and SFLPs into distinct clusters. A majority of the genes in the Si-responsive clusters are highly divergent, but positive selection does not seem to be the driver behind this variability. This study provides a high-resolution map over transcriptional responses to changes in Si availability in T. pseudonana. Hallmark Si-responsive genes are identified, characteristic motifs and domains are classified, and taxonomic and evolutionary implications outlined and discussed. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Nature Publishing Group | nb_NO |
dc.relation.uri | https://www.nature.com/articles/s41598-017-04921-0 | |
dc.title | Dynamic responses to silicon in Thalassiosira pseudonana - Identification, characterisation and classification of signature genes and their corresponding protein motifs | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 14 | nb_NO |
dc.source.volume | 7 | nb_NO |
dc.source.journal | Scientific Reports | nb_NO |
dc.identifier.doi | 10.1038/s41598-017-04921-0 | |
dc.identifier.cristin | 1470392 | |
dc.relation.project | Norges forskningsråd: 238851 | nb_NO |
dc.description.localcode | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.© The Author(s) 2017 | nb_NO |
cristin.unitcode | 194,66,10,0 | |
cristin.unitcode | 194,66,15,0 | |
cristin.unitname | Institutt for biologi | |
cristin.unitname | Institutt for bioteknologi | |
cristin.ispublished | false | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |