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dc.contributor.authorBassett, David
dc.contributor.authorUcar, Seniz
dc.contributor.authorSikorski, Pawel
dc.contributor.authorAndreassen, Jens-Petter
dc.contributor.authorBjørnøy, Sindre Hove
dc.contributor.authorStrand, Berit Løkensgard
dc.identifier.citationActa Biomaterialia. 2016, 44 254-266.nb_NO
dc.description.abstractThe modification of soft hydrogels with hard inorganic components is a method used to form composite materials with application in non-load-bearing bone tissue engineering. The inclusion of an inorganic component may provide mechanical enhancement, introduce osteoconductive or osteoinductive properties, or change other aspects of interactions between native or implanted cells and the material. A thorough understanding of the interactions between such components is needed to improve the rational design of such biomaterials. To achieve this goal, model systems which could allow study of the formation and transformation of mineral phases within a hydrogel network with a range of experimental methods and high spatial and time resolution are needed. Here, we report a detailed investigation of the formation and transformation process of calcium phosphate mineral within an alginate hydrogel matrix. A combination of optical microscopy, confocal Raman microspectroscopy and electron microscopy was used to investigate the spatial distribution, morphology and crystal phase of the calcium phosphate mineral, as well as to study transformation of the mineral phases during the hydrogel mineralization process and upon incubation in a simulated body fluid. It was found, that under the conditions used in this work, mineral initially formed as a metastable amorphous calcium phosphate phase (ACP). The ACP particles had a distinctive spherical morphology and transformed within minutes into brushite in the presence of brushite seed crystals or into octacalcium phosphate, when no seeds were present in the hydrogel matrix. Incubation of brushite–alginate composites in simulated body fluid resulted in formation of hydroxyapatite. The characterization strategy presented here allows for non-destructive, in situ observation of mineralization processes in optically transparent hydrogels with little to no sample preparation.nb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.titleA correlative spatiotemporal microscale study of calcium phosphate formation and transformation within an alginate hydrogel matrix.nb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalActa Biomaterialianb_NO
dc.relation.projectNorges forskningsråd: 214607nb_NO
dc.description.localcodeCopyright © 2017 Elsevier B.V. or its licensors or contributors. This is the authors' accepted and refereed manuscript to the article.nb_NO
cristin.unitnameInstitutt for fysikk
cristin.unitnameInstitutt for bioteknologi
cristin.unitnameInstitutt for kjemisk prosessteknologi

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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal