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dc.contributor.authorSparstad, Torfinn
dc.contributor.authorSharma, Amit Kumar
dc.contributor.authorBones, Atle M.
dc.contributor.authorNymark, Marianne
dc.contributor.authorWinge, Per
dc.date.accessioned2017-01-11T10:39:42Z
dc.date.available2017-01-11T10:39:42Z
dc.date.created2016-04-08T07:55:24Z
dc.date.issued2016
dc.identifier.citationScientific Reports. 2016, 6, 24951nb_NO
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11250/2427015
dc.description.abstractHere we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.nb_NO
dc.language.isoengnb_NO
dc.publisherNature Publishing Groupnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleA CRISPR/Cas9 system adapted for gene editing in marine algaenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.volume6nb_NO
dc.source.journalScientific Reportsnb_NO
dc.identifier.doi10.1038/srep24951
dc.identifier.cristin1349544
dc.description.localcodeThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/nb_NO
cristin.unitcode194,66,10,0
cristin.unitnameInstitutt for biologi
cristin.ispublishedfalse
cristin.fulltextoriginal
cristin.qualitycode1


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