A CRISPR/Cas9 system adapted for gene editing in marine algae
Journal article, Peer reviewed
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Original versionScientific Reports. 2016, 6, 24951 10.1038/srep24951
Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.