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dc.contributor.authorLamb, Jacob Joseph
dc.contributor.authorForfang, Kristin
dc.contributor.authorHohmann-Marriott, Martin Frank
dc.date.accessioned2015-11-25T08:18:36Z
dc.date.accessioned2016-01-05T09:48:32Z
dc.date.available2015-11-25T08:18:36Z
dc.date.available2016-01-05T09:48:32Z
dc.date.issued2015
dc.identifier.citationPLoS ONE 2015, 10(7)nb_NO
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2372554
dc.description.abstractThe fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen temperature (77 K) provides important insights into the organization of the photosynthetic machinery of bacteria and eukaryotes, which cannot be observed at room temperature. Conventionally, to obtain such spectra, a large and costly table-top fluorometer is required. Recently portable, reliable, and largely maintenance-free instruments have become available that can be utilized to accomplish a wide variety of spectroscopy-based measurements in photosynthesis research. In this report, we show how to build such an instrument in order to record 77K fluorescence spectra. This instrument consists of a low power monochromatic light-emitting diode (LED), and a portable CCD array based spectrometer. The optical components are coupled together using a fiber optic cable, and a custom made housing that also supports a dewar flask. We demonstrate that this instrument facilitates the reliable determination of chlorophyll fluorescence emission spectra for the cyanobacterium Synechocystis sp. PCC 6803, and the green alga Chlamydomonas reinhardtii.nb_NO
dc.language.isoengnb_NO
dc.publisherPublic Library of Sciencenb_NO
dc.titleA Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detectornb_NO
dc.typeJournal articlenb_NO
dc.date.updated2015-11-25T08:18:35Z
dc.source.volume10nb_NO
dc.source.journalPLoS ONEnb_NO
dc.source.issue7nb_NO
dc.identifier.doi10.1371/journal.pone.0132258
dc.identifier.cristin1254595
dc.description.localcode© 2015 Lamb et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.nb_NO


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