Blar i Institutt for bioteknologi og matvitenskap på tidsskrift "Microbial Cell Factories"
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A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli
(Journal article; Peer reviewed, 2013)Abstract Background Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often ... -
Activation of chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712 by ethanol shock: Insights from the promoter fusion studies
(Peer reviewed; Journal article, 2016)Background Streptomyces venezuelae ATCC 10712 produces antibiotics chloramphenicol (Cml) and jadomycin (Jad) in response to nutrient limitation and ethanol shock (ES), respectively. Biosynthesis of Cml and Jad was shown ... -
Adaptation of central metabolite pools to variations in growth rate and cultivation conditions in Saccharomyces cerevisiae
(Peer reviewed; Journal article, 2021)Background Saccharomyces cerevisiae is a well-known popular model system for basic biological studies and serves as a host organism for the heterologous production of commercially interesting small molecules and proteins. ... -
Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production
(Journal article; Peer reviewed, 2019)Background Bacteria are widely used as hosts for recombinant protein production due to their rapid growth, simple media requirement and ability to produce high yields of correctly folded proteins. Overproduction of ... -
Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus
(Peer reviewed; Journal article, 2020)Background The suitability of bacteria as microbial cell factories is dependent on several factors such as price of feedstock, product range, production yield and ease of downstream processing. The facultative methylotroph ... -
Increased salt tolerance in Zymomonas mobilis strain generated by adaptative evolution
(Peer reviewed; Journal article, 2020)Background Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol ... -
Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome
(Journal article; Peer reviewed, 2017)Background Through functional screening of a fosmid library, generated from a phytopathogen-suppressive soil metagenome, the novel antifungal chitinase—named Chi18H8 and belonging to family 18 glycosyl hydrolases—was ... -
Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus
(Peer reviewed; Journal article, 2016)Background The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria ... -
Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette
(Journal article; Peer reviewed, 2012)The XylS/Pm expression system has been used to produce recombinant proteins at industrial levels in Escherichia coli. Activation of transcription from the Pm promoter takes place in the presence of benzoic acid or derivatives ...