Browsing NTNU Open by Author "Otterlei, Marit"
Now showing items 1-20 of 32
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5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication
Prikrylova, Terezia; Robertson, Julia; Ferrucci, Francesca; Konorska, Dorota Joanna; Aanes, Håvard; Manaf, Adeel; Zhang, Beibei; Vågbø, Cathrine Broberg; Kusnierczyk, Anna; Gilljam, Karin Margaretha; Løvkvam-Køster, Caroline; Otterlei, Marit; Dahl, John Arne; Enserink, Jorrit; Klungland, Arne; Robertson, Adam Brian (Journal article; Peer reviewed, 2019)In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower ... -
A Study of Loading of Base Excision Repair Proteins at Replication Locus
Bjørås, Karine (Master thesis, 2013)Our genome is constantly exposed to endogenous and exogenous DNA damaging factors. In order to prevent accumulation of mutations and to maintain genome integrity, cells must repair induced lesions. This occurs through a ... -
Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression
Montaldo, Nicola Pietro; Bordin, Diana Lilian; Brambilla, Alessandro; Rösinger, Marcel; Martin, Sarah Fordyce; Bjørås, Karine Øian; Bradamante, Stefano; Aas, Per Arne; Furrer, Antonia; Olsen, Lene Christin; Kunath, Nicolas; Otterlei, Marit; Sætrom, Pål; Bjørås, Magnar; Samson, Leona D.; van Loon, Barbara (Journal article; Peer reviewed, 2019)Base excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases, ... -
Analysis of DNA Repair Proteins and Chromatin Remodeling Factors Using iPOND
Søgaard, Caroline Danielsen (Master thesis, 2012)Most chemotherapeutic treatments rely on induction of severe DNA damage to kill the cancer cells. However, DNA repair pathways can repair the induced lesions, leading to survival of the tumor cells. Thus, inhibition of DNA ... -
Anti-Cancer Potential of Homemade Fresh Garlic Extract Is Related to Increased Endoplasmic Reticulum Stress
Petrovic, Voin; Nepal, Anala; Olaisen, Camilla; Bachke, Siri; Hira, Jonathan; Søgaard, Caroline Krogh; Røst, Lisa Marie; Misund, Kristine; Andreassen, Trygve; Melø, Torun Margareta; Bartosova, Zdenka; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2018)The use of garlic and garlic-based extracts has been linked to decreased incidence of cancer in epidemiological studies. Here we examine the molecular and cellular activities of a simple homemade ethanol-based garlic extract ... -
APIM-Mediated REV3L-PCNA Interaction Important for Error Free TLS Over UV-Induced DNA Lesions in Human Cells
Ræder, Synnøve Brandt; Nepal, Anala; Bjørås, Karine; Seelinger, Mareike; Kolve, Rønnaug Steen; Nedal, Aina; Müller, Rebekka; Otterlei, Marit (Journal article; Peer reviewed, 2018)Proliferating cell nuclear antigen (PCNA) is essential for the organization of DNA replication and the bypass of DNA lesions via translesion synthesis (TLS). TLS is mediated by specialized DNA polymerases, which all interact, ... -
APIM-peptide targeting PCNA improves the efficacy of docetaxel treatment in the TRAMP mouse model of prostate cancer
Søgaard, Caroline Danielsen; Moestue, Siver Andreas; Rye, Morten Beck; Kim, Jana; Nepal, Anala; Liabakk, Nina-Beate; Bachke, Siri; Bathen, Tone Frost; Otterlei, Marit; Hill, Deborah Katherine (Journal article; Peer reviewed, 2018)Docetaxel is the chemotherapeutic choice for metastatic hormone-refractory prostate cancer, however, it only marginally improves the survival rate. The purpose of the present study was to examine if a peptide ... -
Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
Petrovic, Voin; Olaisen, Camilla; Sharma, Animesh; Nepal, Anala; Bugge, Steffen; Sundby, Eirik; Hoff, Bård Helge; Slupphaug, Geir; Otterlei, Marit (Journal article; Peer reviewed, 2017)Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins ... -
Cytosolic scaffold functions of PCNA
Olaisen, Camilla (Doctoral theses at NTNU;2017:203, Doctoral thesis, 2017)PCNA som et plattform-protein i cytosol Kroppen vår består av millioner av celler som alle stammer fra en enkelt celle. For hver celledeling må arvestoffet, DNA-et, kopieres feilfritt i et samarbeid mellom mange ulike ... -
Dynamics of base excision repair proteins; recruitment to replication forks and DNA lesions
Bjørås, Karine Øian (Doctoral theses at NTNU;2017:275, Doctoral thesis, 2017)DNA er arvestoffet vårt og finnes i de fleste celler i kroppen. DNA er satt sammen av to tråder bestående av sukkermolekyler, fosfat og nitrogenbaser (nukleotider). Disse kjemiske strukturene blir kontinuerlig utsatt for ... -
Effects of APIM-containing peptides on the regulation of the PI3K/Akt and MEK/ERK pathways
Hira, Jonathan (Master thesis, 2013)PI3K/Akt and MEK/ERK pathways are important cellular signaling system which controls many vital cellular processes, including cell survival, proliferation, differentiation and migration. These pathways are often deregulated ... -
Effects of Hypoxia on Cell Growth and Metabolism in the Leukemia Cell Line JJN-3 and Bladder Cancer Cell Line UM-UC-3
Langørgen, Ida Eide (Master thesis, 2018)Hypoxia is a condition in which the oxygen (O2) access is extremely reduced and thus limits the O2 available for cellular respiration. The hypoxic condition induces metabolic adjustments in the cells that permit further ... -
Enhanced efficacy of bleomycin in bladder cancer cells by photochemical internalization
Baglo, Yan; Hagen, Lars; Høgset, Anders; Drabløs, Finn; Otterlei, Marit; Gederaas, Odrun Arna (Journal article; Peer reviewed, 2014)Bleomycin is a cytotoxic chemotherapeutic agent widely used in cancer treatment. However, its efficacy in different cancers is low, possibly due to limited cellular internalization. In this study, a novel approach known ... -
Examination of different APIM-variants and APIM's role in TRF2 protection of the telomere ends
Talgø, Nina Magnusson (Master thesis, 2013)Most chemotherapeutic drugs induce DNA damage to the cells. However, the toxicity of DNA damaging drugs can be reduced by DNA repair mechanisms, and the cancer cells can survive despite the damage. Therefore, by targeting ... -
Examinations of the functionality of APIM in TRF2 and the importance for telomere stability
Kjelstrup, Hanna (Master thesis, 2014)Maintaining genome integrity is crucial for life, and therefore, complex control and repair mechanisms have evolved. Because cells are especially susceptible to damage during replication, many DNA repair pathways are coupled ... -
Helicase-Like Transcription Factor HLTF and E3 Ubiquitin Ligase SHPRH Confer DNA Damage Tolerance through Direct Interactions with Proliferating Cell Nuclear Antigen (PCNA)
Otterlei, Marit; Seelinger, Mareike (Journal article; Peer reviewed, 2020)To prevent replication fork collapse and genome instability under replicative stress, DNA damage tolerance (DDT) mechanisms have evolved. The RAD5 homologs, HLTF (helicase-like transcription factor) and SHPRH (SNF2, ... -
Human ALKBH4 Interacts with Proteins Associated with Transcription
Bjørnstad, Linn; Meza, Trine Johansen; Otterlei, Marit; Olafsrud, Solveig Mjelstad; Meza-Zepeda, Leonardo Andrés; Falnes, Pål (Journal article; Peer reviewed, 2012)The Fe(II)- and 2-oxoglutarate (2OG)-dependent dioxygenase AlkB from E. coli is a demethylase which repairs alkyl lesions in DNA, as well as RNA, through a direct reversal mechanism. Humans possess nine AlkB homologs ... -
The human RAD5 homologs, HLTF and SHPRH, have separate functions in DNA damage tolerance dependent on the DNA lesion type
Otterlei, Marit; Seelinger, Mareike; Søgaard, Caroline Krogh (Peer reviewed; Journal article, 2020)Helicase-like transcription factor (HLTF) and SNF2, histone-linker, PHD and RING finger domain-containing helicase (SHPRH), the two human homologs of yeast Rad5, are believed to have a vital role in DNA damage tolerance ... -
Increased Anticancer Efficacy of Intravesical Mitomycin C Therapy when combined with a PCNA Targeting Peptide
Gederaas, Odrun Arna; Søgaard, Caroline Danielsen; Viset, Trond; Bachke, Siri; Bruheim, Per; Arum, Carl-Jørgen; Otterlei, Marit (Journal article; Peer reviewed, 2014)Non–muscle-invasive bladder cancers (NMIBCs) are tumors confined to the mucosa or the mucosa/submucosa. An important challenge in treatment of NMIBC is both high recurrence and high progression rates. Consequently, more ... -
Is APIM functional in RAD5 homolog HLTF?
Tviberg, Heidi Lind (Master thesis, 2016)To ensure secure transmission of genome to daughter cells, all cells carry out highly processive error-free replication of the genome. Proliferating cell nuclear antigen (PCNA) plays a central part as processivity factor ...