Blar i NTNU Open på forfatter "Otterlei, Marit"
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5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication
Prikrylova, Terezia; Robertson, Julia; Ferrucci, Francesca; Konorska, Dorota Joanna; Aanes, Håvard; Manaf, Adeel; Zhang, Beibei; Vågbø, Cathrine Broberg; Kusnierczyk, Anna; Gilljam, Karin Margaretha; Løvkvam-Køster, Caroline; Otterlei, Marit; Dahl, John Arne; Enserink, Jorrit; Klungland, Arne; Robertson, Adam Brian (Journal article; Peer reviewed, 2019)In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower ... -
A Study of Loading of Base Excision Repair Proteins at Replication Locus
Bjørås, Karine (Master thesis, 2013)Our genome is constantly exposed to endogenous and exogenous DNA damaging factors. In order to prevent accumulation of mutations and to maintain genome integrity, cells must repair induced lesions. This occurs through a ... -
Activating the Cpx response induces tolerance to antisense PNA delivered by an arginine-rich peptide in Escherichia coli
Frimodt-Møller, Jakob; Koulouktsis, Andreas; Charbon, Godefroid; Otterlei, Marit; Nielsen, Peter E.; Løbner-Olesen, Anders (Peer reviewed; Journal article, 2021)Cell-penetrating peptides (CPPs) are increasingly used for cellular drug delivery in both pro- and eukaryotic cells, and oligoarginines have attracted special attention. How arginine-rich CPPs translocate across the cell ... -
Activation of multiple stress responses in Staphylococcus aureus substantially lowers the minimal inhibitory concentration when combining two novel antibiotic drug candidates
Singleton, Amanda Holstad; Bergum, Olaug Elisabeth Torheim; Søgaard, Caroline Krogh; Røst, Lisa M.; Olsen, Cecilie Elisabeth; Blindheim, Fredrik Heen; Ræder, Synnøve Brandt; Bjørnstad, Frithjof Andreas; Sundby, Eirik; Hoff, Bård Helge; Bruheim, Per; Otterlei, Marit (Peer reviewed; Journal article, 2023)The past few decades have been plagued by an increasing number of infections caused by antibiotic resistant bacteria. To mitigate the rise in untreatable infections, we need new antibiotics with novel targets and drug ... -
Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression
Montaldo, Nicola Pietro; Bordin, Diana Lilian; Brambilla, Alessandro; Rösinger, Marcel; Martin, Sarah Fordyce; Bjørås, Karine Øian; Bradamante, Stefano; Aas, Per Arne; Furrer, Antonia; Olsen, Lene Christin; Kunath, Nicolas; Otterlei, Marit; Sætrom, Pål; Bjørås, Magnar; Samson, Leona D.; van Loon, Barbara (Journal article; Peer reviewed, 2019)Base excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases, ... -
Analysis of DNA Repair Proteins and Chromatin Remodeling Factors Using iPOND
Søgaard, Caroline Danielsen (Master thesis, 2012)Most chemotherapeutic treatments rely on induction of severe DNA damage to kill the cancer cells. However, DNA repair pathways can repair the induced lesions, leading to survival of the tumor cells. Thus, inhibition of DNA ... -
Anti-Cancer Potential of Homemade Fresh Garlic Extract Is Related to Increased Endoplasmic Reticulum Stress
Petrovic, Voin; Nepal, Anala; Olaisen, Camilla; Bachke, Siri; Hira, Jonathan; Søgaard, Caroline Krogh; Røst, Lisa Marie; Misund, Kristine; Andreassen, Trygve; Melø, Torun Margareta; Bartosova, Zdenka; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2018)The use of garlic and garlic-based extracts has been linked to decreased incidence of cancer in epidemiological studies. Here we examine the molecular and cellular activities of a simple homemade ethanol-based garlic extract ... -
Antimicrobial peptides targeting the beta-clamp
Nepal, Anala (Doctoral theses at NTNU;2023:94, Doctoral thesis, 2023) -
APIM-Mediated REV3L-PCNA Interaction Important for Error Free TLS Over UV-Induced DNA Lesions in Human Cells
Ræder, Synnøve Brandt; Nepal, Anala; Bjørås, Karine; Seelinger, Mareike; Kolve, Rønnaug Steen; Nedal, Aina; Müller, Rebekka; Otterlei, Marit (Journal article; Peer reviewed, 2018)Proliferating cell nuclear antigen (PCNA) is essential for the organization of DNA replication and the bypass of DNA lesions via translesion synthesis (TLS). TLS is mediated by specialized DNA polymerases, which all interact, ... -
APIM-peptide targeting PCNA improves the efficacy of docetaxel treatment in the TRAMP mouse model of prostate cancer
Søgaard, Caroline Danielsen; Moestue, Siver Andreas; Rye, Morten Beck; Kim, Jana; Nepal, Anala; Liabakk, Nina-Beate; Bachke, Siri; Bathen, Tone Frost; Otterlei, Marit; Hill, Deborah Katherine (Journal article; Peer reviewed, 2018)Docetaxel is the chemotherapeutic choice for metastatic hormone-refractory prostate cancer, however, it only marginally improves the survival rate. The purpose of the present study was to examine if a peptide ... -
ATX-101, a Peptide Targeting PCNA, Has Antitumor Efficacy Alone or in Combination with Radiotherapy in Murine Models of Human Glioblastoma
Gravina, Giovanni Luca; Colapietro, Alessandro; Andrea, Mancini; Rossetti, Alessandra; Martelucci, Stefano; Ventura, Luca; Di Franco, Martina; Marampon, Francesco; Mattei, Vinvenzo; Biordi, Leda Assunta; Otterlei, Marit; Festuccia, Claudio (Peer reviewed; Journal article, 2022)Cell proliferation requires the orchestrated actions of a myriad of proteins regulating DNA replication, DNA repair and damage tolerance, and cell cycle. Proliferating cell nuclear antigen (PCNA) is a master regulator which ... -
Bladder cancer from bench to bedside: Sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
Blindheim, Augun Jodis (Doctoral theses at NTNU;2021:72, Doctoral thesis, 2021) -
Broad-Spectrum Antibacterial Peptide Kills Extracellular and Intracellular Bacteria Without Affecting Epithelialization
Nepal, Anala; Ræder, Synnøve Brandt; Søgaard, Caroline Krogh; Haugan, Maria Schei; Otterlei, Marit (Peer reviewed; Journal article, 2021)New antibacterial drugs with novel modes of action are urgently needed as antibiotic resistance in bacteria is increasing and spreading throughout the world. In this study, we aimed to explore the possibility of using ... -
Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
Petrovic, Voin; Olaisen, Camilla; Sharma, Animesh; Nepal, Anala; Bugge, Steffen; Sundby, Eirik; Hoff, Bård Helge; Slupphaug, Geir; Otterlei, Marit (Journal article; Peer reviewed, 2017)Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins ... -
Cytosolic scaffold functions of PCNA
Olaisen, Camilla (Doctoral theses at NTNU;2017:203, Doctoral thesis, 2017)PCNA som et plattform-protein i cytosol Kroppen vår består av millioner av celler som alle stammer fra en enkelt celle. For hver celledeling må arvestoffet, DNA-et, kopieres feilfritt i et samarbeid mellom mange ulike ... -
Development and Optimization of a Screen for Determining Reduced Sensitivity to the Antimicrobial Peptide Betatide in 4,000 Non-Essential Single Gene Knockouts of Escherichia coli
Johnsen, Norun (Master thesis, 2022)Abstract will be available on 2075-07-01 -
Differentially expressed extracellular vesicle-contained micrornas before and after transurethral resection of bladder tumors
Strømme, Olaf; Heck, Kathleen; Brede, Gaute; Lindholm, Håvard Takle; Otterlei, Marit; Arum, Carl-Jørgen (Peer reviewed; Journal article, 2021)Bladder cancer (BC) is currently diagnosed and monitored by cystoscopy, a costly and invasive procedure. Potential biomarkers in urine, blood, and, more recently, extracellular vesicles (EVs), have been explored as ... -
DNA damage tolerance in human cells mediated by the APIM-containing proteins REV3L, HLTF, and SHPRH
Seelinger, Mareike (Doctoral theses at NTNU;2020:108, Doctoral thesis, 2020) -
Dynamics of base excision repair proteins; recruitment to replication forks and DNA lesions
Bjørås, Karine Øian (Doctoral theses at NTNU;2017:275, Doctoral thesis, 2017)DNA er arvestoffet vårt og finnes i de fleste celler i kroppen. DNA er satt sammen av to tråder bestående av sukkermolekyler, fosfat og nitrogenbaser (nukleotider). Disse kjemiske strukturene blir kontinuerlig utsatt for ... -
Effects of APIM-containing peptides on the regulation of the PI3K/Akt and MEK/ERK pathways
Hira, Jonathan (Master thesis, 2013)PI3K/Akt and MEK/ERK pathways are important cellular signaling system which controls many vital cellular processes, including cell survival, proliferation, differentiation and migration. These pathways are often deregulated ...