Blar i NTNU Open på forfatter "Otterlei, Marit"
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Dynamics of base excision repair proteins; recruitment to replication forks and DNA lesions
Bjørås, Karine Øian (Doctoral theses at NTNU;2017:275, Doctoral thesis, 2017)DNA er arvestoffet vårt og finnes i de fleste celler i kroppen. DNA er satt sammen av to tråder bestående av sukkermolekyler, fosfat og nitrogenbaser (nukleotider). Disse kjemiske strukturene blir kontinuerlig utsatt for ... -
Effects of APIM-containing peptides on the regulation of the PI3K/Akt and MEK/ERK pathways
Hira, Jonathan (Master thesis, 2013)PI3K/Akt and MEK/ERK pathways are important cellular signaling system which controls many vital cellular processes, including cell survival, proliferation, differentiation and migration. These pathways are often deregulated ... -
Effects of Hypoxia on Cell Growth and Metabolism in the Leukemia Cell Line JJN-3 and Bladder Cancer Cell Line UM-UC-3
Langørgen, Ida Eide (Master thesis, 2018)Hypoxia is a condition in which the oxygen (O2) access is extremely reduced and thus limits the O2 available for cellular respiration. The hypoxic condition induces metabolic adjustments in the cells that permit further ... -
Enhanced efficacy of bleomycin in bladder cancer cells by photochemical internalization
Baglo, Yan; Hagen, Lars; Høgset, Anders; Drabløs, Finn; Otterlei, Marit; Gederaas, Odrun Arna (Journal article; Peer reviewed, 2014)Bleomycin is a cytotoxic chemotherapeutic agent widely used in cancer treatment. However, its efficacy in different cancers is low, possibly due to limited cellular internalization. In this study, a novel approach known ... -
Examination of different APIM-variants and APIM's role in TRF2 protection of the telomere ends
Talgø, Nina Magnusson (Master thesis, 2013)Most chemotherapeutic drugs induce DNA damage to the cells. However, the toxicity of DNA damaging drugs can be reduced by DNA repair mechanisms, and the cancer cells can survive despite the damage. Therefore, by targeting ... -
Examinations of the functionality of APIM in TRF2 and the importance for telomere stability
Kjelstrup, Hanna (Master thesis, 2014)Maintaining genome integrity is crucial for life, and therefore, complex control and repair mechanisms have evolved. Because cells are especially susceptible to damage during replication, many DNA repair pathways are coupled ... -
Halogenated Pyrrolopyrimidines with Low MIC on Staphylococcus aureus and Synergistic Effects with an Antimicrobial Peptide
Olsen, Cecilie Elisabeth; Blindheim, Fredrik Heen; Søgaard, Caroline Krogh; Røst, Lise Marie; Singleton, Amanda; Bergum, Olaug Elisabeth Torheim; Bruheim, Per; Otterlei, Marit; Sundby, Eirik; Hoff, Bård Helge (Peer reviewed; Journal article, 2022)Currently, there is a world-wide rise in antibiotic resistance causing burdens to individuals and public healthcare systems. At the same time drug development is lagging behind. Therefore, finding new ways of treating ... -
Helicase-Like Transcription Factor HLTF and E3 Ubiquitin Ligase SHPRH Confer DNA Damage Tolerance through Direct Interactions with Proliferating Cell Nuclear Antigen (PCNA)
Otterlei, Marit; Seelinger, Mareike (Journal article; Peer reviewed, 2020)To prevent replication fork collapse and genome instability under replicative stress, DNA damage tolerance (DDT) mechanisms have evolved. The RAD5 homologs, HLTF (helicase-like transcription factor) and SHPRH (SNF2, ... -
Human ALKBH4 Interacts with Proteins Associated with Transcription
Bjørnstad, Linn; Meza, Trine Johansen; Otterlei, Marit; Olafsrud, Solveig Mjelstad; Meza-Zepeda, Leonardo Andrés; Falnes, Pål (Journal article; Peer reviewed, 2012)The Fe(II)- and 2-oxoglutarate (2OG)-dependent dioxygenase AlkB from E. coli is a demethylase which repairs alkyl lesions in DNA, as well as RNA, through a direct reversal mechanism. Humans possess nine AlkB homologs ... -
The human RAD5 homologs, HLTF and SHPRH, have separate functions in DNA damage tolerance dependent on the DNA lesion type
Otterlei, Marit; Seelinger, Mareike; Søgaard, Caroline Krogh (Peer reviewed; Journal article, 2020)Helicase-like transcription factor (HLTF) and SNF2, histone-linker, PHD and RING finger domain-containing helicase (SHPRH), the two human homologs of yeast Rad5, are believed to have a vital role in DNA damage tolerance ... -
Identification of Potential Cellular Pathways Involved in Mediating the Rapid Bactericidal Effects of the Novel β-clamp-targeting Antimicrobial Peptide Betatide
Wang, Emmy (Master thesis, 2023)Økende antimikrobiell resistens, samt redusert utvikling av nye antibiotika, har ført til en global utfordring som innebærer en stor økonomisk og sosial belastning. Betatide er et nytt antimikrobielt peptid som binder til ... -
Increased Anticancer Efficacy of Intravesical Mitomycin C Therapy when combined with a PCNA Targeting Peptide
Gederaas, Odrun Arna; Søgaard, Caroline Danielsen; Viset, Trond; Bachke, Siri; Bruheim, Per; Arum, Carl-Jørgen; Otterlei, Marit (Journal article; Peer reviewed, 2014)Non–muscle-invasive bladder cancers (NMIBCs) are tumors confined to the mucosa or the mucosa/submucosa. An important challenge in treatment of NMIBC is both high recurrence and high progression rates. Consequently, more ... -
Is APIM functional in RAD5 homolog HLTF?
Tviberg, Heidi Lind (Master thesis, 2016)To ensure secure transmission of genome to daughter cells, all cells carry out highly processive error-free replication of the genome. Proliferating cell nuclear antigen (PCNA) plays a central part as processivity factor ... -
Mass Spectrometric Metabolic Profiling of the Human Bladder Cancer Cell Line UM-UC-3 under Cytostatic Stress
Forsland, Yngve (Master thesis, 2016)Muscle Invasive Bladder Cancers (MIBCs) contributes to approximately 30% of bladder cancer at initial diagnosis in patients, accompanied by an only 50% five-year survival rate. The standard procedure for treating this type ... -
Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication.
Bjørås, Karine; Sousa, Mirta; Sharma, Animesh; Fonseca, Davi de Miranda; Søgaard, Caroline Danielsen; Bjørås, Magnar; Otterlei, Marit (Journal article; Peer reviewed, 2017)Base lesions in DNA can stall the replication machinery or induce mutations if bypassed. Consequently, lesions must be repaired before replication or in a post-replicative process to maintain genomic stability. Base excision ... -
Novel Peptides Targeting the β-Clamp Rapidly Kill Planktonic and Biofilm Staphylococcus epidermidis Both in vitro and in vivo
Ræder, Synnøve Brandt; Sandbakken, Erik Thorvaldsen; Nepal, Anala; Løseth, Kirsti; Bergh, Kåre; Witsø, Eivind; Otterlei, Marit (Peer reviewed; Journal article, 2021)Antimicrobial resistance is an increasing threat to global health and challenges the way we treat infections. Peptides containing the PCNA interacting motif APIM (APIM-peptides) were recently shown to bind to the bacterial ... -
Nucleoside Analogues Are Potent Inducers of Pol V-mediated Mutagenesis
Sumabe, Balagra Kasim; Ræder, Synnøve Brandt; Røst, Lisa M.; Sharma, Animesh; Donkor, Eric S.; Mosi, Lydia; Duodu, Samuel; Bruheim, Per; Otterlei, Marit (Peer reviewed; Journal article, 2021)Drugs targeting DNA and RNA in mammalian cells or viruses can also affect bacteria present in the host and thereby induce the bacterial SOS system. This has the potential to increase mutagenesis and the development of ... -
Nucleoside Analogues Are Potent Inducers of Pol V-mediated Mutagenesis
Sumabe, Balagra Kasim; Ræder, Synnøve Brandt; Røst, Lisa M.; Sharma, Animesh; Donkor, Eric S.; Mosi, Lydia; Duodu, Samuel; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2021)Drugs targeting DNA and RNA in mammalian cells or viruses can also affect bacteria present in the host and thereby induce the bacterial SOS system. This has the potential to increase mutagenesis and the development of ... -
Nucleotide excision repair is associated with the replisome and its efficiency depends on a direct interaction between XPA and PCNA
Gilljam, Karin Margaretha; Müller, Rebekka; Liabakk, Nina-Beate; Otterlei, Marit (Journal article; Peer reviewed, 2012)Proliferating cell nuclear antigen (PCNA) is an essential protein for DNA replication, DNA repair, cell cycle regulation, chromatin remodeling, and epigenetics. Many proteins interact with PCNA through the PCNA interacting ... -
P38 MAPK signaling and phosphorylations in the BRCT1 domain regulate XRCC1 recruitment to sites of DNA damage
Sousa, Mirta; Bjørås, Karine; Hanssen-Bauer, Audun; Solvang-Garten, Karin; Otterlei, Marit (Journal article; Peer reviewed, 2017)XRCC1 is a scaffold protein involved in base excision repair and single strand break repair. It is a phosphoprotein that contains more than 45 phosphorylation sites, however only a few of these have been characterized and ...