• 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication 

      Prikrylova, Terezia; Robertson, Julia; Ferrucci, Francesca; Konorska, Dorota Joanna; Aanes, Håvard; Manaf, Adeel; Zhang, Beibei; Vågbø, Cathrine Broberg; Kusnierczyk, Anna; Gilljam, Karin Margaretha; Løvkvam-Køster, Caroline; Otterlei, Marit; Dahl, John Arne; Enserink, Jorrit; Klungland, Arne; Robertson, Adam Brian (Journal article; Peer reviewed, 2019)
      In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower ...
    • A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA 

      Robertson, Adam Brian; Dahl, John Arne; Vågbø, Cathrine Broberg; Tripathi, P; Krokan, Hans Einar; Klungland, Arne (Journal article; Peer reviewed, 2011)
      Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in ...
    • A transgenic minipig model of Huntington’s disease shows early signs of behavioral and molecular pathologies 

      Askeland, Georgina; Rodinova, Marie; Štufková, Hana; Dosoudilova, Zaneta; Baxa, Monika; Smatlikova, Petra; Bohuslavova, Bozena; Klempir, Jiri; Nguyen, The Duong; Kuśnierczyk, Anna; Bjørås, Magnar; Klungland, Arne; Hansikova, Hana; Ellederova, Zdenka; Eide, Lars (Journal article; Peer reviewed, 2018)
      Huntington's disease (HD) is a monogenic, progressive, neurodegenerative disorder with currently no available treatment. The Libechov transgenic minipig model for HD (TgHD) displays neuroanatomical similarities to humans ...
    • ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA 

      Van den Born, Erwin; Vågbø, Cathrine Broberg; Songe-Møller, Lene; Leihne, VIbeke; Lien, Guro Flor; Leszczynska, G; Malkiewicz, A; Krokan, Hans Einar; Kirpekar, F; Klungland, Arne; Falnes, Pål (Journal article; Peer reviewed, 2011)
      Mammals have nine different homologues (ALKBH1–9) of the Escherichia coli DNA repair demethylase AlkB. ALKBH2 is a genuine DNA repair enzyme, but the in vivo function of the other ALKBH proteins has remained elusive. It ...
    • Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism 

      Weber, Alain R.; Krawczyk, Claudia; Robertson, Adam Brian; Kusnierczyk, Anna; Vågbø, Cathrine Broberg; Schürmann, David; Klungland, Arne; Schär, Primo (Peer reviewed; Journal article, 2016)
      Cytosine methylation in CpG dinucleotides is an epigenetic DNA modification dynamically established and maintained by DNA methyltransferases and demethylases. Molecular mechanisms of active DNA demethylation began to surface ...
    • Changes of 5-hydroxymethylcytosine distribution during myeloid and lymphoid differentiation of CD34+ cells 

      Tekpli, Xavier; Urbanucci, Alfonso; Hashim, Adnan; Vågbø, Cathrine Broberg; Lyle, Robert; Kringen, Marianne K.; Staff, Anne Cathrine; Dybedal, Ingunn; Mills, Ian Geoffrey; Klungland, Arne; Staerk, Judith (Journal article; Peer reviewed, 2016)
      Background Hematopoietic stem cell renewal and differentiation are regulated through epigenetic processes. The conversion of 5-methylcytosine into 5-hydroxymethylcytosine (5hmC) by ten-eleven-translocation enzymes provides ...
    • DNA base modifications in honey bee and fruit fly genomes suggest an active demethylation machinery with species- and tissue-specific turnover rates 

      Rasmussen, Erik; Vågbø, Cathrine Broberg; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne; Amdam, Gro Vang; Dahl, John Arne (Journal article; Peer reviewed, 2016)
      Well-known epigenetic DNA modifications in mammals include the addition of a methyl group and a hydroxyl group to cytosine, resulting in 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) respectively. In contrast, ...
    • Excision of the doubly methylated base N4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins 

      Alexeeva, Marina; Guragain, Prashanna; Tesfahun, Almaz Nigatu; Tomkuvienė, Miglė; Arshad, Aysha; Gerasimaitė, Rūta; Rukšėnaitė, Audronė; Urbanavičiūtė, Giedrė; Bjørås, Magnar; Lærdahl, Jon Kristen; Klungland, Arne; Klimašauskas, Saulius; Bjelland, Svein (Journal article; Peer reviewed, 2018)
      Cytosine (C) in DNA is often modified to 5-methylcytosine (m5C) to execute important cellular functions. Despite the significance of m5C for epigenetic regulation in mammals, damage to m5C has received little attention. ...
    • Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation 

      Landfors, Miriam; Johansen, Jostein; Aronsen, Jan Magnus; Vågbø, Cathrine Broberg; Doré, Louis C.; He, Chuan; Sjaastad, Ivar; Sætrom, Pål; Fedorcsák, Péter; Dahl, John Arne; Aanes, Håvard; Fusser, Markus; Klungland, Arne (Journal article; Peer reviewed, 2017)
      Sertoli cells have dual roles during the cells’ lifetime. In the juvenile mammal, Sertoli cells proliferate and create the structure of the testis, and during puberty they cease to proliferate and take on the adult role ...
    • Histone methylations define neural stem/progenitor cell subtypes in the mouse subventricular zone 

      Zhang, Zhichao; Manaf, Adeel; Li, Yanjiao; Pérez, Sonia Peña; Suganthan, Rajikala; Dahl, John Arne; Bjørås, Magnar; Klungland, Arne (Journal article; Peer reviewed, 2019)
      Neural stem/progenitor cells (NSPCs) persist in the mammalian brain throughout life and can be activated in response to the physiological and pathophysiological stimuli. Epigenetic reprogramming of NPSC represents a novel ...
    • Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients 

      Askeland, Georgina; Dosoudilova, Zaneta; Rodinova, Marie; Klempir, Jiri; Liskova, Irena; Kusnierczyk, Anna; Bjørås, Magnar; Nesse, Gaute; Klungland, Arne; Hansikova, Hana; Eide, Lars (Journal article; Peer reviewed, 2018)
      Huntington’s disease (HD) is a progressive neurodegenerative disorder primarily affecting the basal ganglia and is caused by expanded CAG repeats in the huntingtin gene. Except for CAG sizing, mitochondrial and nuclear DNA ...
    • Metabolism and DNA repair shape a specific modification pattern in mitochondrial DNA 

      Pawar, Tina; Bjørås, Magnar; Klungland, Arne; Eide, Lars (Journal article, 2017)
      The mitochondrial DNA (mtDNA) resides in the vicinity of energy-rich reactions. Thus, chemical modifications of mtDNA might mirror mitochondrial processes and could serve as biomarkers of metabolic processes in the ...
    • NEIL3-Dependent Regulation of Cardiac Fibroblast Proliferation Prevents Myocardial Rupture 

      Olsen, Maria Belland; Hildrestrand, Gunn Annette; Scheffler, Katja; Vinge, Leif Erik; Alfsnes, Katrine; Palibrk, Vuk; Wang, Junbai; Neurauter, Christine Gran; Gomez, Luisa Fernanda Luna; Johansen, Jostein; Øgaard, Jonas; Ohm, Ingrid Kristine; Slupphaug, Geir; Kusnierczyk, Anna; Fiane, Arnt E; Brorson, Sverre Henning; Zhang, Lili; Gullestad, Lars; Louch, William Edward; Iversen, Per Ole; Østlie, Ingunn; Klungland, Arne; Christensen, Geir Arve; Sjaastad, Ivar; Sætrom, Pål; Yndestad, Arne; Aukrust, Pål; Bjørås, Magnar; Finsen, Alexandra (Journal article; Peer reviewed, 2017)
      Myocardial infarction (MI) triggers a reparative response involving fibroblast proliferation and differentiation driving extracellular matrix modulation necessary to form a stabilizing scar. Recently, it was shown that a ...
    • No cancer predisposition or increased spontaneous mutation frequencies in NEIL DNA glycosylases-deficient mice 

      Rolseth, Veslemøy; Luna, Luisa; Olsen, Ann-Karin; Suganthan, Rajikala; Scheffler, Katja; Neurauter, Christine Gran; Esbensen, Qin Ying; Kusnierczyk, Anna; Hildrestrand, Gunn Annette; Graupner, Anne; Andersen, Jill Mari; Slupphaug, Geir; Klungland, Arne; Nilsen, Hilde; Bjørås, Magnar (Journal article; Peer reviewed, 2017)
      Base excision repair (BER) is a major pathway for removal of DNA base lesions and maintenance of genomic stability, which is essential in cancer prevention. DNA glycosylases recognize and remove specific lesions in the ...
    • OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation 

      Yang, Mingyi; Lin, Xiaolin; Segers, Filip; Suganthan, Rajikala; Hildrestrand, Gunn Annette; Rinholm, Johanne Egge; Aas, Per Arne; Sousa, Mirta; Holm, Sverre; Bolstad, Nils; Warren, David; Berge, Rolf Kristian; Johansen, Rune Forstrøm; Yndestad, Arne; Kristiansen, Elise; Klungland, Arne; Gomez, Luisa Fernanda Luna; Eide, Lars; Halvorsen, Bente; Aukrust, Pål; Bjørås, Magnar (Peer reviewed; Journal article, 2020)
      Oxidation resistance gene 1 (OXR1) protects cells against oxidative stress. We find that male mice with brain-specific isoform A knockout (Oxr1A−/−) develop fatty liver. RNA sequencing of male Oxr1A−/− liver indicates ...
    • Protozoan ALKBH8 Oxygenases Display both DNA Repair and tRNA Modification Activities 

      Zdzalik, Daria; Vågbø, Cathrine Broberg; Kirpekar, F; Davydova, Erna; Puścian, Alicja; Maciejewska, Agnieszka M.; Krokan, Hans Einar; Klungland, Arne; Tudek, Barbara; Van den Born, Erwin; Falnes, Pål (Journal article; Peer reviewed, 2014)
      The ALKBH family of Fe(II) and 2-oxoglutarate dependent oxygenases comprises enzymes that display sequence homology to AlkB from E. coli, a DNA repair enzyme that uses an oxidative mechanism to dealkylate methyl and etheno ...
    • Synergistic Actions of Ogg1 and Mutyh DNA Glycosylases Modulate Anxiety-like Behavior in Mice 

      Bjørge, Monica Dahl; Hildrestrand, Gunn Annette; Scheffler, Katja; Suganthan, Rajikala; Rolseth, Veslemøy; Kusnierczyk, Anna; Rowe, Alexander D.; Vågbø, Cathrine Broberg; Vetlesen, Susanne; Eide, Lars; Slupphaug, Geir; Yusaku, Nakabeppu; Timothy, Bredy; Klungland, Arne; Bjørås, Magnar (Journal article; Peer reviewed, 2015)
      Ogg1 and Mutyh DNA glycosylases cooperate to prevent mutations caused by 8-oxoG, a major premutagenic DNA lesion associated with cognitive decline. We have examined behavior and cognitive function in mice deficient of these ...
    • The DNA dioxygenase ALKBH2 protects Arabidopsis thaliana against methylation damage 

      Meza, Trine Johansen; Moen, Marivi; Vågbø, Cathrine Broberg; Krokan, Hans Einar; Klungland, Arne; Grini, Paul Eivind; Falnes, Pål (Journal article; Peer reviewed, 2012)
      The Escherichia coli AlkB protein (EcAlkB) is a DNA repair enzyme which reverses methylation damage such as 1-methyladenine (1-meA) and 3-methylcytosine (3-meC). The mammalian AlkB homologues ALKBH2 and ALKBH3 display ...
    • Ythdf2-mediated m6A mRNA clearance modulates neural development in mice 

      Li, Miaomiao; Zhao, Xu; Wang, Wei; Shi, Hailing; Pan, Qingfei; Lu, Zhike; Perez, Sonia Peña; Suganthan, Rajikala; He, Chuan; Bjørås, Magnar; Klungland, Arne (Journal article; Peer reviewed, 2018)
      Background N 6 -methyladenosine (m6A) modification in mRNAs was recently shown to be dynamically regulated, indicating a pivotal role in multiple developmental processes. Most recently, it was shown that the Mettl3-Mettl14 ...