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Excision of the doubly methylated base N4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins

Alexeeva, Marina; Guragain, Prashanna; Tesfahun, Almaz Nigatu; Tomkuvienė, Miglė; Arshad, Aysha; Gerasimaitė, Rūta; Rukšėnaitė, Audronė; Urbanavičiūtė, Giedrė; Bjørås, Magnar; Lærdahl, Jon Kristen; Klungland, Arne; Klimašauskas, Saulius; Bjelland, Svein
Journal article, Peer reviewed
Published version
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Alexeeva (Locked)
URI
http://hdl.handle.net/11250/2592470
Date
2018
Metadata
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  • Institutt for klinisk og molekylær medisin [2066]
  • Publikasjoner fra CRIStin - NTNU [20734]
Original version
10.1098/rstb.2017.0337
Abstract
Cytosine (C) in DNA is often modified to 5-methylcytosine (m5C) to execute important cellular functions. Despite the significance of m5C for epigenetic regulation in mammals, damage to m5C has received little attention. For instance, almost no studies exist on erroneous methylation of m5C by alkylating agents to doubly or triply methylated bases. Owing to chemical evidence, and because many prokaryotes express methyltransferases able to convert m5C into N4,5-dimethylcytosine (mN4,5C) in DNA, mN4,5C is probably present in vivo. We screened a series of glycosylases from prokaryotic to human and found significant DNA incision activity of the Escherichia coli Nei and Fpg proteins at mN4,5C residues in vitro. The activity of Nei was highest opposite cognate guanine followed by adenine, thymine (T) and C. Fpg-complemented Nei by exhibiting the highest activity opposite C followed by lower activity opposite T. To our knowledge, this is the first description of a repair enzyme activity at a further methylated m5C in DNA, as well as the first alkylated base allocated as a Nei or Fpg substrate. Based on our observed high sensitivity to nuclease S1 digestion, we suggest that mN4,5C occurs as a disturbing lesion in DNA and that Nei may serve as a major DNA glycosylase in E. coli to initiate its repair.
Publisher
The Royal Society
Journal
Philosophical Transactions of the Royal Society of London. Biological Sciences

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