Browsing NTNU Open by Author "Stokke, Bjørn Torger"
Now showing items 41-60 of 64
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Nanoscale Structure and Spectroscopic Probing of Aβ1-40 Fibril Bundle Formation
Psonka-Antonczyk, Katarzyna Maria; Hammarström, Per; Johansson, Leif B. G.; Lindgren, Mikael; Stokke, Bjørn Torger; Nilsson, K. Peter R.; Nyström, Sofie (Peer reviewed; Journal article, 2016)Amyloid plaques composed of fibrillar Amyloid-β (Aβ) are hallmarks of Alzheimer's disease. However, Aβ fibrils are morphologically heterogeneous. Conformation sensitive luminescent conjugated oligothiophenes (LCOs) are ... -
Nanoscopic and Photonic Ultrastructural Characterization of Two Distinct Insulin Amyloid States
Psonka-Antonczyk, Katarzyna Maria; Duboisset, Julien; Stokke, Bjørn Torger; Zako, Tamotsu; Kobayashi, Takahiro; Maeda, Mizuo; Nyström, Sofie; Mason, Jeffrey; Hammarström, Per; Nilsson, K. Peter R.; Lindgren, Mikael (Journal article; Peer reviewed, 2012)Two different conformational isoforms or amyloid strains of insulin with different cytotoxic capacity have been described previously. Herein these filamentous and fibrillar amyloid states of insulin were investigated using ... -
NNanomechanical characteristics of proteins and peptides in amyloid
Boayue, Nya Mehnwolo (Master thesis, 2012)......The understanding of the aggregation of amyloid fibrils is essential as they are linked to a number of diseases such as Alzheimer and Parkston’s disease. Amy- loids from different proteins or peptides have common ... -
Novel imaging technologies for characterization of microbial extracellular polysaccharides
Lilledahl, Magnus Borstad; Stokke, Bjørn Torger (Journal article; Peer reviewed, 2015)Understanding of biology is underpinned by the ability to observe structures at various length scales. This is so in a historical context and is also valid today. Evolution of novel insight often emerges from technological ... -
On the determination of mechanical properties of aqueous microgels-towards high-throughput characterization
Øvreeide, Ingrid Haga; Szydlak, Renata; Luty, Marcin; Ahmed, Husnain; Prot, Victorien Emile; Skallerud, Bjørn Helge; Zemła, Joanna; Lekka, Małgorzata; Stokke, Bjørn Torger (Journal article; Peer reviewed, 2021)Aqueous microgels are distinct entities of soft matter with mechanical signatures that can be different from their macroscopic counterparts due to confinement effects in the preparation, inherently made to consist of more ... -
A Perturbation Analysis Approach for Studying the Effect of Swelling Kinetics on Instabilities in Hydrogel Plates
Ilseng, Arne; Skallerud, Bjørn Helge; Stokke, Bjørn Torger; Prot, Victorien Emile (Journal article; Peer reviewed, 2021)The onset of surface instability during diffusion-driven swelling of hydrogels depends on the kinetics of the swelling process. Here, we outline a perturbation analysis framework using a finite difference approach for ... -
Plasma Treatment of PDMS for Microcontact Printing (μCP) of Lectins Decreases Silicone Transfer and Increases the Adhesion of Bladder Cancer Cells
Zemła, Joanna; Szydlak, Renata; Gajos, Katarzyna; Kozłowski, Łukasz; Zieliński, Tomasz; Luty, Marcin; Øvreeide, Ingrid Haga; Prot, Victorien Emile; Stokke, Bjørn Torger; Lekka, Małgorzata (Peer reviewed; Journal article, 2023)The present study investigates silicone transfer occurring during microcontact printing (μCP) of lectins with polydimethylsiloxane (PDMS) stamps and its impact on the adhesion of cells. Static adhesion assays and single-cell ... -
Polymer sequencing by molecular machines: a framework for predicting the resolving power of a sliding contact force spectroscopy sequencing method
Dunlop, Alex; Bowman, Kate; Aarstad, Olav Andreas; Skjåk-Bræk, Gudmund; Stokke, Bjørn Torger; Round, Andrew N (Journal article, 2017)We evaluate an AFM-based single molecule force spectroscopy method for mapping sequences in otherwise difficult to sequence heteropolymers, including glycosylated proteins and glycans. The sliding contact force spectroscopy ... -
Production and Application of Micronsized Polysaccharide Particles - Studying Perturbation of a Model Mucus Barrier with Total Internal Reflection Fluorescence (TIRF) Microscopy and Atomic Force Microscopy (AFM) Indentation
Myklatun, Ahne (Master thesis, 2011)The overall aim of this project was to produce homogeneously sized polysaccharide microparticles and apply these and similar sized particles as probes for investigation of mucin layers as a model for a biological barrier. ... -
Recovering fluorophore concentration profiles from confocal images near lateral refractive index step changes
Parelius Jonasova, Eleonora; Bjørkøy, Astrid; Stokke, Bjørn Torger (Peer reviewed; Journal article, 2016)Optical aberrations due to refractive index mismatches occur in various types of microscopy due to refractive differences between the sample and the immersion fluid or within the sample. We study the effects of lateral ... -
Responsive Hydrogels for Label-Free Signal Transduction within Biosensors
Gawel, Kamila; Barriet, David; Sletmoen, Marit; Stokke, Bjørn Torger (Journal article; Peer reviewed, 2010)Hydrogels have found wide application in biosensors due to their versatile nature. This family of materials is applied in biosensing either to increase the loading capacity compared to two-dimensional surfaces, or to support ... -
Single-Molecule Pair Studies of RGD-Coupled Alginate and Integrin
Lee, Hye Won (Master thesis, 2014) -
Spatiotemporal mapping of cascading processes in oligonucleotide-functionalized responsive hydrogels
Jonášová, Eleonóra Parelius (Doctoral theses at NTNU;2020:102, Doctoral thesis, 2020)Hydrogels – crosslinked polymer networks holding a large amount of water – have many properties that make them useful for medical applications, such as their resemblance to biological tissues, biocompatibility and ... -
Surface Acoustic Wave Acoustophoresis for Microfluidic Based Micron and Submicron Particle Separation
Kavli, Ole-Andreas Kvivik (Master thesis, 2016)Microvesicles secreted from our cells referred to as exosomes contain vital information on our health status and are believed to revolutionize early diagnosis. Therefore, separation of such particles is a growing field ... -
Swelling Dynamics of a DNA-Polymer Hybrid Hydrogel Prepared Using Polyethylene Glycol as a Porogen
Gao, Ming; Gawel, Kamila; Stokke, Bjørn Torger (Peer reviewed; Journal article, 2015)DNA-polyacrylamide hybrid hydrogels designed with covalent and double-stranded (dsDNA) crosslinks respond to specific single-stranded DNA (ssDNA) probes by adapting new equilibrium swelling volume. The ssDNA probes need ... -
Swelling of a hemi-ellipsoidal ionic hydrogel for determination of material properties of deposited thin polymer films: an inverse finite element approach
Prot, Victorien Emile; Sveinsson, Hrafn Mar; Gawel, Kamila; Gao, Ming; Skallerud, Bjørn Helge; Stokke, Bjørn Torger (Journal article; Peer reviewed, 2013)Selective deposition of polymers at the surface of an ionic hydrogel is conventionally used to tailor properties of the composite material for application in for instance drug release and cell encapsulation. Here we describe ... -
Swelling properties of bioresponsive hydrogels: Swelling kinetics, and transport mechanisms in a hydrogel for signal transducing
Ratnavel, Inthu (Master thesis, 2013)An oligonucleotide grafted acrylamide-bis-acrylamide based hydrogel was tested as a signal transducer. The sensing strand was conjugated with Iowa black that quenched the signal from the Alexa fluor 488 conjugated at the ... -
Synergy of condensing agents and macromolecular crowding on DNA condensation
Ramisetty, Sravani Keerthi (Doctoral theses at NTNU;2017:334, Doctoral thesis, 2017)Imagine the packing of 100,000 meters of very thin copper wire inside a basketball!!! To add more complexity to the problem, imagine that the wire is highly negatively charged, causing repulsion. Further, consider quickly ... -
The Characterisation and Quantification of Immobilised Concanavalin A on Quartz Surfaces Based on The Competitive Binding to Glucose and Fluorescent Labelled Dextran
Hoang, Trinh Bich; Stokke, Bjørn Torger; Hanke, Ulrik; Johannessen, Agne; Johannessen, Erik Andrew (Journal article; Peer reviewed, 2019)The competition between various carbohydrates in the binding to Concanavalin A (Con A) can be exploited in gravimetric microsensors that detect changes in mass or viscoelasticity as a function of glucose concentration. ... -
A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalised Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution
Wong, Che Fai Alex; Van Vliet, L.; Bhujbal, Swapnil Vilas; Guo, Chengzhi; Sletmoen, Marit; Stokke, Bjørn Torger; Hollfelder, Florian; Lale, Rahmi (Journal article; Peer reviewed, 2021)Water-in-oil emulsion droplets are an attractive format for ultrahigh-throughput screening in functional metagenomics and directed evolution applications that allow libraries with more than 107 members to be characterized ...