Spatiotemporal mapping of cascading processes in oligonucleotide-functionalized responsive hydrogels
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- Institutt for fysikk 
Hydrogels – crosslinked polymer networks holding a large amount of water – have many properties that make them useful for medical applications, such as their resemblance to biological tissues, biocompatibility and biodegradability. So-called responsive hydrogels are also able to change their properties in response to a change in their surroundings. Some of their potential applications include manufacturing of sensors, delivering drugs to a specific location in the body, or serving as scaffolds for tissue engineering. The aim of the thesis was to investigate the processes that lead to swelling of oligonucleotide-functionalized hydrogels which respond to the presence of a target DNA or other oligonucleotide. An understanding of these processes and how changing one affects the rest is important in order to be able to adjust the hydrogels to their intended applications. The swelling of the hydrogels and the transport of the DNA within them were observed using interferometry. Then the binding strength of the target to the network was varied to investigate how the transport and the overall swelling are affected.
Has partsPaper 1: Parelius Jonasova, Eleonora; Stokke, Bjørn Torger. Bioresponsive DNA-co-polymer hydrogels for fabrication of sensors. Current Opinion in Colloid & Interface Science 2016 ;Volum 26. s. 1-8 https://doi.org/10.1016/j.cocis.2016.07.001
Paper 2: Parelius Jonasova, Eleonora; Bjørkøy, Astrid; Stokke, Bjørn Torger. Recovering fluorophore concentration profiles from confocal images near lateral refractive index step changes. Journal of Biomedical Optics 2016 ;Volum 21.(12) https://doi.org/10.1117/1.JBO.21.12.126014
Paper 3: Jonasova, Eleonora Parelius; Bjørkøy, Astrid; Stokke, Bjørn Torger. Toehold length of target ssDNA affects reaction-diffusion behavior in DNA-responsive DNA-co-acrylamide hydrogels. Biomacromolecules 2020 ;Volum 21.(5) s. 1687-1699 https://doi.org/10.1021/acs.biomac.9b01515 Copyright © 2019 American Chemical Society
Paper 4: Jonasova, Eleonora Parelius; Stokke, Bjørn Torger. Morpholino target molecular properties affect the swelling process of oligomorpholino-functionalized responsive hydrogels. Polymers 2020 ;Volum 12.(2) https://doi.org/10.3390/polym12020268 This is an open access article distributed under the Creative Commons Attribution License (CC BY 4.0)