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dc.contributor.authorWang, Bingduo
dc.contributor.authorWöhler, Aliona
dc.contributor.authorGreven, Johannes
dc.contributor.authorSalzmann, Rebekka J. S.
dc.contributor.authorKeller, Cindy M.
dc.contributor.authorTertel, Tobias
dc.contributor.authorZhao, Qun
dc.contributor.authorMert, Ümit
dc.contributor.authorHorst, Klemens
dc.contributor.authorLupu, Ludmila
dc.contributor.authorHuber-Lang, Markus
dc.contributor.authorvan Griensven, Martijn
dc.contributor.authorMollnes, Tom Erik
dc.contributor.authorSchaaf, Sebastian
dc.contributor.authorSchwab, Robert
dc.contributor.authorStrassburg, Christian P.
dc.contributor.authorSchmidt-Wolf, Ingo G. H.
dc.contributor.authorGiebel, Bernd
dc.contributor.authorHildebrand, Frank
dc.contributor.authorLukacs-Kornek, Veronika
dc.contributor.authorWillms, Arnulf G.
dc.contributor.authorKornek, Miroslaw T.
dc.identifier.citationFrontiers in Immunology. 2023, 14 .en_US
dc.description.abstractBackground Despite major advances in medicine, blood-borne biomarkers are urgently needed to support decision-making, including polytrauma. Here, we assessed serum-derived extracellular vesicles (EVs) as potential markers of decision-making in polytrauma. Objective Our Liquid Biopsy in Organ Damage (LiBOD) study aimed to differentiate polytrauma with organ injury from polytrauma without organ injury. We analysed of blood-borne small EVs at the individual level using a combination of immunocapture and high-resolution imaging. Methods To this end, we isolated, purified, and characterized small EVs according to the latest Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines from human blood collected within 24 h post-trauma and validated our results using a porcine polytrauma model. Results We found that small EVs derived from monocytes CD14 + and CD14 + CD61 + were significantly elevated in polytrauma with organ damage. To be precise, our findings revealed that CD9 + CD14 + and CD14 + CD61 + small EVs exhibited superior performance compared to CD9 + CD61 + small EVs in accurately indicating polytrauma with organ damage, reaching a sensitivity and a specificity of 0.81% and 0.97%, respectively. The results in humans were confirmed in an independent porcine model of polytrauma. Conclusion These findings suggest that these specific types of small EVs may serve as valuable, non-invasive, and objective biomarkers for assessing and monitoring the severity of polytrauma and associated organ damage.en_US
dc.publisherFrontiers Mediaen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleLiquid Biopsy in Organ Damage: small extracellular vesicle chip-based assessment of polytraumaen_US
dc.title.alternativeLiquid Biopsy in Organ Damage: small extracellular vesicle chip-based assessment of polytraumaen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.source.journalFrontiers in Immunologyen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal